Zalata A, Elhanbly S, Abdalla H, Serria M S, Aziz A, El-Dakrooy S A, El-Bakary A A, Mostafa T
Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Andrologia. 2014 Dec;46(10):1141-7. doi: 10.1111/and.12206. Epub 2013 Dec 16.
Cypermethrin, a type II synthetic pyrethroid pesticide, is widely used in pest control programmes in agriculture and public health. This study aimed to assess the potential effect of cypermethrin on human spermatozoa and the possible ameliorative effects of vitamins C and E. Semen samples of 20 healthy normozoospermic men were divided into six aliquots at room temperature. The first aliquot served as control not exposed to treatments, and the second was incubated with 20 mm vit. C and 2 mm vit. E where the third one was exposed to 10 μm cypermethrin for 6 h. The other three aliquots were incubated with vit. C, vit. E and both vitamins for 30 min before cypermethrin exposure. Semen aliquots were analysed for sperm motility, sperm viability, hypo-osmotic swelling test and modified alkaline comet assay. The results demonstrated a significant decrease in sperm motion, sperm function and increased sperm DNA damage in the cypermethrin group. Addition of vitamins C and E alone/combined led to significant improvement in sperm motion, sperm function and DNA damage, being maximal with both vitamins together. It is concluded that in vitro cypermethrin can alter sperm function and induce DNA damage in spermatozoa, which is improved after using vitamins C and E.
氯氰菊酯是一种II型合成拟除虫菊酯类杀虫剂，广泛应用于农业和公共卫生领域的害虫防治项目。本研究旨在评估氯氰菊酯对人类精子的潜在影响以及维生素C和维生素E可能的改善作用。将20名健康的正常精子男性的精液样本在室温下分成六等份。第一等份作为未接受处理的对照，第二等份与20 mmol维生素C和2 mmol维生素E一起孵育，第三等份暴露于10 μmol氯氰菊酯中6小时。另外三等份在暴露于氯氰菊酯之前与维生素C、维生素E以及两种维生素一起孵育30分钟。对精液样本进行精子活力、精子存活率、低渗肿胀试验和改良碱性彗星试验分析。结果表明，氯氰菊酯组精子运动、精子功能显著降低，精子DNA损伤增加。单独添加维生素C和维生素E/联合添加导致精子运动、精子功能和DNA损伤显著改善，两种维生素一起添加时改善效果最佳。得出结论：体外氯氰菊酯可改变精子功能并诱导精子DNA损伤，使用维生素C和维生素E后这种损伤得到改善。
