Askari H A, Check J H, Peymer N, Bollendorf A
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden.
Arch Androl. 1994 Jul-Aug;33(1):11-5. doi: 10.3109/01485019408987797.
Excessive generation of reactive oxygen species and increased peroxidation of the phospholipids in the membrane is proposed as a biochemical basis for the reduced activity of spermatozoa in cryopreserved semen. The possible role of alpha-tocopherol (VE) and ascorbic acid (VC) in improving the quality of frozen semen was investigated. Semen samples were divided into aliquots and frozen in freezing media with and without VC or VE. Sperm parameters, including motility after thawing and also at 2 h, % grade A motility (rapid and linear forward progression), and hypoosmotic swelling (HOS) scores, were measured in all samples. Vitamin C did not result in any improvement in sperm parameters studies. Vitamin E improved the post-thaw motility minimally. This improvement, although statistically significant, was actually very small. There were, however, wide variations between the samples. The motility improvement was considered in 5 of 12 samples, and HOS improved in some of the samples tested with VE. Future studies are needed to determine if these antioxidants can produce a consistent improvement in some individuals rather than help the majority of patients.
活性氧的过度生成以及膜中磷脂过氧化作用的增强被认为是冷冻精液中精子活力降低的生化基础。研究了α-生育酚（维生素E）和抗坏血酸（维生素C）在改善冷冻精液质量方面的可能作用。精液样本被分成小份，分别在含有和不含有维生素C或维生素E的冷冻介质中冷冻。对所有样本测量了精子参数，包括解冻后以及解冻后2小时的活力、A级活力百分比（快速直线前向运动）和低渗肿胀（HOS）评分。维生素C并未使所研究的精子参数有任何改善。维生素E使解冻后活力稍有提高。尽管这一改善在统计学上具有显著性，但实际上非常小。然而，样本之间存在很大差异。12个样本中有5个样本的活力有所改善，并且在一些用维生素E测试的样本中低渗肿胀有所改善。需要进一步的研究来确定这些抗氧化剂是否能在某些个体中产生持续的改善，而不是帮助大多数患者。
