Mendelsohn R, Brauner J W, Gericke A
Annu Rev Phys Chem. 1995;46:305-34. doi: 10.1146/annurev.pc.46.100195.001513.
The theory and practice of external infrared reflection absorption spectrometry (IRRAS) as applied to monomolecular films at the air-water interface are reviewed. The observed IR frequencies for films of amphiphilic species provide information about the conformational states of the hydrocarbon chains and the hydrogen bonding and ionization states of the polar head groups, under conditions of controlled surface pressure. Determination of molecular orientation is also feasible and requires detailed consideration of the reflection-absorption properties of the three- phase (air-monolayer-water) system. Current theoretical approaches are described. Applications of IRRAS to the study of single- and double-chain amphiphiles and proteins are reviewed, and initial excursions into biochemistry (interfacial enzyme catalysis) and physiology (pulmonary surfactant function) are reported.
本文综述了外反射红外吸收光谱法（IRRAS）应用于气-水界面单分子膜的理论与实践。在可控表面压力条件下，两亲性物质膜的红外频率观测值可提供有关烃链构象状态以及极性头部基团氢键和电离状态的信息。分子取向的测定也是可行的，这需要详细考虑三相（空气-单分子层-水）系统的反射吸收特性。文中描述了当前的理论方法。对IRRAS在单链和双链两亲性物质及蛋白质研究中的应用进行了综述，并报道了其在生物化学（界面酶催化）和生理学（肺表面活性剂功能）方面的初步探索。
