Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Oncol Res. 2013;21(1):15-21. doi: 10.3727/096504013X13786659070235.
Adiponectin, an adipocyte-derived hormone with anti-inflammatory and antitumor activity, inhibits esophageal adenocarcinoma (EAC) cell proliferation and induces apoptosis. Chronic inflammation is a key process involved in initiation and progression of EAC, but the roles and mechanisms of adiponectin in inflammation have not been fully understood in EAC. We aimed to analyze the effects of two types of adiponectin, full-length adiponectin (f-Ad) and globular adiponectin (g-Ad), on inflammatory factors' expression and explore the roles of ROS/NF-κB signaling pathway in adiponectin-regulated inflammation in EAC cells. It was found that f-Ad and g-Ad differently regulated both mRNA and protein levels of TNF-α, IL-8, and IL-6 in a dose-dependent manner in OE19 cells. g-Ad apparently induced TNF-α, IL-8, and IL-6 production, which was inhibited by PDTC or NAC, and increased intracellular ROS levels and NF-κB p65 activation, whereas f-Ad significantly suppressed production of inflammatory factors and NF-κB p65 activation and also decreased the intracellular ROS levels. In conclusion, the study demonstrated that g-Ad exerts a proinflammatory effect whereas f-Ad appears to induce an anti-inflammatory effect in a ROS/NF-κB-dependent manner in OE19 cells.
脂联素是一种脂肪细胞来源的具有抗炎和抗肿瘤活性的激素，它能抑制食管腺癌（EAC）细胞增殖并诱导细胞凋亡。慢性炎症是 EAC 发生和发展的关键过程，但脂联素在 EAC 中的炎症作用及其机制尚未完全阐明。我们旨在分析两种类型的脂联素，全长脂联素（f-Ad）和球形脂联素（g-Ad），对 OE19 细胞中炎症因子表达的影响，并探讨 ROS/NF-κB 信号通路在脂联素调节 EAC 细胞炎症中的作用。结果发现，f-Ad 和 g-Ad 以剂量依赖的方式不同地调节 OE19 细胞中 TNF-α、IL-8 和 IL-6 的 mRNA 和蛋白水平。g-Ad 明显诱导 TNF-α、IL-8 和 IL-6 的产生，这种产生被 PDTC 或 NAC 抑制，并增加细胞内 ROS 水平和 NF-κB p65 激活，而 f-Ad 则显著抑制炎症因子的产生和 NF-κB p65 激活，并降低细胞内 ROS 水平。总之，该研究表明，g-Ad 发挥促炎作用，而 f-Ad 则以 ROS/NF-κB 依赖的方式在 OE19 细胞中诱导抗炎作用。
