Research Laboratory for Beverage Technology, Kirin Company Limited, Technovillage 3F, 1-17-1 Namamugi, Tsurumi-ku, Yokohama, Kanagawa 230-8628, Japan.
Research Laboratory for Beverage Technology, Kirin Company Limited, Technovillage 3F, 1-17-1 Namamugi, Tsurumi-ku, Yokohama, Kanagawa 230-8628, Japan.
J Biosci Bioeng. 2014 May;117(5):617-9. doi: 10.1016/j.jbiosc.2013.10.024. Epub 2013 Dec 9.
In order to increase the value of freshwater algae as raw ingredients for health foods and feed for seawater-based farmed fish, we sought to breed high-chlorophyll halotolerant Chlorella with the objective of generating strains with both high chlorophyll concentrations (≥ 5%) and halotolerance (up to 1% NaCl). We used the Chlorella vulgaris K strain in our research institute culture collection and induced mutations with UV irradiation and acriflavine which is known to effect mutations of mitochondrial DNA that are associated with chlorophyll production. Screenings were conducted on seawater-based "For Chlorella spp." (FC) agar medium, and dark-green-colored colonies were visually selected by macroscopic inspection. We obtained a high-chlorophyll halotolerant strain (designated C. vulgaris M-207A7) that had a chlorophyll concentration of 6.7% (d.m.), a level at least three-fold higher than that of K strain. This isolate also exhibited a greater survival rate in seawater that of K strain.
为了提高淡水藻类作为健康食品原料和海水养殖鱼类饲料的价值，我们试图培育高叶绿素耐盐性小球藻，目标是生成既具有高叶绿素浓度（≥5%）又具有耐盐性（高达 1%NaCl）的菌株。我们在研究所的培养物收集库中使用了普通小球藻 K 株，并使用紫外线和吖啶黄素诱导突变，已知吖啶黄素会影响与叶绿素生成相关的线粒体 DNA 的突变。在基于海水的“用于小球藻属”（FC）琼脂培养基上进行筛选，并通过宏观检查目视选择深绿色的菌落。我们获得了一株高叶绿素耐盐性菌株（命名为 C. vulgaris M-207A7），其叶绿素浓度为 6.7%（干重），至少比 K 株高三倍。该分离株在海水中的存活率也高于 K 株。
