Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Faculty of Science and Engineering, Soka University, 1-236 Tangi-machi, Hachioji City, Tokyo 192-8577, Japan.
Mar Drugs. 2020 Jul 17;18(7):367. doi: 10.3390/md18070367.
The commercial cultivation of microalgae began in the 1960s and was one of the first target organisms. The species has long been considered a potential source of renewable energy, an alternative for phytoremediation, and more recently, as a growth and immune stimulant. However, which is one of the most studied microalga, has never been comprehensively profiled chemically. In the present study, comprehensive profiling of the metabolome grown under normal culture conditions was carried out, employing tandem LC-MS/MS to profile the ethanolic extract and GC-MS for fatty acid analysis. The fatty acid profile of was shown to be rich in omega-6, -7, -9, and -13 fatty acids, with omega-6 being the highest, representing more than sixty percent (>60%) of the total fatty acids. This is a clear indication that this species of could serve as a good source of nutrition when incorporated in diets. The profile also showed that the main fatty acid composition was that of C-C (>92%), suggesting that it might be a potential candidate for biodiesel production. LC-MS/MS analysis revealed carotenoid constituents comprising violaxanthin, neoxanthin, lutein, β-carotene, vulgaxanthin I, astaxanthin, and antheraxanthin, along with other pigments such as the chlorophylls. In addition to these, amino acids, vitamins, and simple sugars were also profiled, and through mass spectrometry-based molecular networking, 48 phospholipids were putatively identified.
微藻的商业种植始于 20 世纪 60 年代，是最早的目标生物之一。长期以来，该物种一直被认为是可再生能源的潜在来源，是植物修复的替代品，最近又被认为是一种生长和免疫刺激物。然而，作为研究最多的微藻之一，其化学成分从未得到全面描述。在本研究中，采用串联 LC-MS/MS 对醇提物进行代谢组学分析，GC-MS 对脂肪酸进行分析，对正常培养条件下生长的进行了全面的代谢组学分析。结果表明，的脂肪酸谱富含ω-6、ω-7、ω-9 和 ω-13 脂肪酸，其中 ω-6 含量最高，占总脂肪酸的 60%以上。这清楚地表明，这种微藻可以作为营养物质添加到饮食中。该图谱还表明，主要脂肪酸组成为 C-C（>92%），这表明它可能是生物柴油生产的潜在候选者。LC-MS/MS 分析揭示了类胡萝卜素成分，包括 violaxanthin、neoxanthin、lutein、β-胡萝卜素、vulgaxanthin I、astaxanthin 和 antheraxanthin，以及其他色素，如叶绿素。除了这些，还对氨基酸、维生素和简单糖进行了分析，并通过基于质谱的分子网络分析，推测出 48 种磷脂。
