Departments of Internal Medicine, Division of Diabetes, Endocrinology, and Metabolism, Omaha, NE, United States; Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, United States; Research Services, VA Nebraska Western Iowa Health Care System, Omaha, NE, United States.
Research Services, VA Nebraska Western Iowa Health Care System, Omaha, NE, United States; Departments of Internal Medicine, Division of Diabetes, Endocrinology, and Metabolism, Omaha, NE, United States.
Prostaglandins Leukot Essent Fatty Acids. 2014 Jan;90(1):13-21. doi: 10.1016/j.plefa.2013.10.002. Epub 2013 Nov 7.
The objective of this study was to determine the effects of enrichment with n-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on the differentiation of 3T3-L1 preadipocytes. Enrichment with DHA but not EPA significantly increased the differentiation markers compared to control differentiated cells. DHA compared to EPA treatment led to a greater increase in adiponectin secretion and, conditioned media collected from DHA treated cells inhibited monocyte migration. Moreover, DHA treatment resulted in inhibition of pro-inflammatory signaling pathways. DHA treated cells predominantly accumulated DHA in phospholipids whereas EPA treatment led to accumulation of both EPA and its elongation product docosapentaenoic acid (DPA), an n-3 fatty acid. Of note, adding DPA to DHA inhibited DHA-induced differentiation. The differential effects of EPA and DHA on preadipocyte differentiation may be due, in part, to differences in their intracellular modification which could impact the type of n-3 fatty acids incorporated into the cells.
本研究旨在确定富含 n-3 脂肪酸（二十碳五烯酸（EPA）和二十二碳六烯酸（DHA））对 3T3-L1 前体脂肪细胞分化的影响。与对照分化细胞相比，DHA 的富集而非 EPA 的富集显著增加了分化标志物。与 EPA 处理相比，DHA 处理导致脂联素分泌增加，并且从 DHA 处理的细胞收集的条件培养基抑制单核细胞迁移。此外，DHA 处理导致促炎信号通路的抑制。DHA 处理的细胞主要在磷脂中积累 DHA，而 EPA 处理导致 EPA 及其延伸产物二十二碳五烯酸（DPA）的积累，DPA 是一种 n-3 脂肪酸。值得注意的是，添加 DPA 抑制了 DHA 诱导的分化。EPA 和 DHA 对前体脂肪细胞分化的不同影响可能部分归因于它们在细胞内修饰方面的差异，这可能会影响掺入细胞的 n-3 脂肪酸的类型。
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