从进口到德国的比利时猪中分离到的“Brachyspira hampsonii” clade I。
"Brachyspira hampsonii" clade I isolated from Belgian pigs imported to Germany.
机构信息
Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine, Hannover, Germany.
Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine, Hannover, Germany.
出版信息
Vet Microbiol. 2014 Jan 31;168(2-4):432-5. doi: 10.1016/j.vetmic.2013.11.016. Epub 2013 Nov 22.
This report describes the detection of "Brachyspira (B.) hampsonii" clade I in Belgian pigs imported to Germany. Two of seventeen pigs from one herd were reported positive for Brachyspira hyodysenteriae by culture in a Belgian diagnostic laboratory, but negative for this Brachyspira species by specific PCR. In this study, from 22 fecal samples and 2 colon contents of these animals various Brachyspira species were cultured and identified by nox-RFLP as Brachyspira murdochii, Brachyspira innocens and Brachyspira intermedia. Albeit the six B. intermedia isolates proved to be negative in a species specific PCR. Sequencing of the nox-gene of three of these isolates revealed that the sequences were 99% identical to published sequences of "B. hampsonii" clade I. From one pig which was positive for "B. hampsonii" clade I histopathology was done and showed moderate lesions consistent with brachyspiral disease.
本报告描述了在德国进口的比利时猪中检测到“Brachyspira (B.) hampsonii”I 群。一家比利时诊断实验室的培养结果显示，一个畜群的 17 头猪中有 2 头为“Brachyspira hyodysenteriae”阳性，但特定 PCR 检测结果为阴性。在这项研究中，从这些动物的 22 份粪便样本和 2 份结肠内容物中，通过nox-RFLP 培养和鉴定了各种 Brachyspira 种，分别为 Brachyspira murdochii、Brachyspira innocens 和 Brachyspira intermedia。尽管 6 株 B. intermedia 分离株在种特异性 PCR 中呈阴性。对其中 3 株分离株的 nox 基因进行测序表明，这些序列与已发表的“B. hampsonii”I 群序列 99%一致。对一株“B. hampsonii”I 群阳性的猪进行了组织病理学检查，结果显示中度病变与短螺旋体病一致。
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