Departamento de Zootecnia, Universidad Autónoma de Baja California Sur, Km. 5.5 carr. al Sur. A.P. 19-B, C.P. 23080 La Paz, B.C.S., Mexico.
Centro de Investigaciones Biológicas del Noroeste, S.C. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, C.P. 23096 La Paz, B.C.S., Mexico.
Vet Parasitol. 2014 Feb 24;200(1-2):225-8. doi: 10.1016/j.vetpar.2013.11.018. Epub 2013 Nov 26.
Two studies were carried out in order to test the effects of neem tree extracts (Azadirachta indica A. Juss) on sheep bot fly larvae (Oestrus ovis L. Diptera: Oestridae). First, aqueous extracts from neem seeds (ASNE) at 0, 5 y 10% (w/v) concentrations were tested on larval mortality in vitro. In a second study, the effect of oral administration with neem seed meal (0, 100 y 200mg/kg) and neem leaves (1% of diet) on number of larvae found at necropsy and larval development was evaluated in experimentally O. ovis-infected sheep. Results in Experiment 1 showed a significant (P<0.05) effect of ASNE on time to L1 mortality in a dosis-dependent manner. In Experiment 2, oral administration of seeds or leaves did not affect the number of larvae found at necropsy of the sheep, but interfered with larval development and there was a tendency to reduce larval weight at the end of the infection period (55d).
进行了两项研究，以测试印楝树提取物（Azadirachta indica A. Juss）对绵羊狂蝇幼虫（Oestrus ovis L. 双翅目：狂蝇科）的影响。首先，在体外测试了浓度为 0、5 和 10%（w/v）的印楝树种子水提物（ASNE）对幼虫死亡率的影响。在第二项研究中，口服给予印楝树种子粉（0、100 和 200mg/kg）和印楝树叶（饮食的 1%）对剖检时发现的幼虫数量和幼虫发育的影响在实验性感染 O. ovis 的绵羊中进行了评估。实验 1 的结果表明，ASNE 对 L1 死亡率的时间有显著（P<0.05）的剂量依赖性影响。在实验 2 中，种子或叶子的口服给药并不影响绵羊剖检时发现的幼虫数量，但干扰了幼虫的发育，并且在感染期结束时（55d）有降低幼虫体重的趋势。
