USDA Arid Land Agricultural Research Center, 21881 N. Cardon Lane, Maricopa, AZ 85238, USA.
J Insect Physiol. 2014 Jan;60:104-10. doi: 10.1016/j.jinsphys.2013.12.001. Epub 2013 Dec 11.
Newly mated females of the plant bug, Lygus hesperus Knight, enter a refractory period during which their sexual receptivity to courting males is greatly reduced for several days. This behavioral change appears to be induced by male-derived factors delivered in the spermatophore during copulation. To better understand the source of the factor(s) responsible for the inhibition, the homogenates of spermatophores, or of the individual organs that provide the constituents of the spermatophore, were injected directly into the abdomen of virgin females. The contents of the lateral and medial accessory glands both appear to produce inhibitory effects, but those of the seminal vesicle had no effect. Treatment of the homogenate also indicated that the active factor(s) is heat labile and water soluble. Several unique proteins were found in the water soluble fraction of the spermatophore, one of which is similar in size to the Drosophila melanogaster sex peptide, a male derived compound known to inhibit receptivity in female flies. In addition, spermatophores contained a substantial quantity of juvenile hormone, a key endocrine regulator of reproductive behavior and physiology in most insects. The results support the hypothesized role of males in manipulating the post-mating behavior of females, and suggest this is achieved through multiple components that act in concert to induce both short- and long-term effects.
新交配的植食蝽，Lygus hesperus Knight，进入不应期，在此期间它们对求偶雄性的性接受能力大大降低了数天。这种行为变化似乎是由交配过程中雄虫在精囊中传递的雄性衍生因素引起的。为了更好地了解负责抑制的因素的来源，精囊或提供精囊成分的各个器官的匀浆直接注入处女雌虫的腹部。侧附腺和中附腺的内容物似乎都产生了抑制作用，但精囊的内容物没有作用。处理匀浆也表明，活性因子是热不稳定和水溶性的。在精囊的水溶性部分发现了几种独特的蛋白质，其中一种与果蝇（Drosophila melanogaster）性肽大小相似，性肽是一种已知的雄性衍生化合物，可抑制雌性苍蝇的接受能力。此外，精囊中含有大量的保幼激素，这是大多数昆虫生殖行为和生理学的关键内分泌调节剂。结果支持了雄性在操纵雌性交配后行为的假设作用，并表明这是通过协同作用的多个成分来实现的，这些成分既能产生短期效应，也能产生长期效应。
