Brent Colin S, Miyasaki Katelyn, Vuong Connor, Miranda Brittany, Steele Bronwen, Brent Kristoffer G, Nath Rachna
United States Department of Agriculture, Arid Land Agricultural Research Center, Agricultural Research Service, 21881 North Cardon Lane, Maricopa, AZ, 85138, USA.
Desert Vista High School, Phoenix, AZ, USA.
J Comp Physiol B. 2016 Feb;186(2):169-79. doi: 10.1007/s00360-015-0953-1. Epub 2015 Dec 19.
Mating induces behavioral and physiological changes in the plant bug Lygus hesperus Knight (Hemiptera: Miridae). After receiving seminal products, which include the systemic regulator juvenile hormone (JH), females enter a post-mating period lasting several days during which they enhance their oviposition rate and lose interest in remating. To elucidate the regulation of these behavioral changes in L. hesperus, biogenic amines were quantified in the heads of females at 5 min, 1 h and 24 h after copulation and compared to levels in virgins using high-performance liquid chromatography coupled with electrochemical detection. Mating significantly increased dopamine (DA) after 1 and 24 h, and decreased octopamine (OA) after 5 min and 1 h. Serotonin did not change with mating, but tyramine was significantly reduced after 5 min. While injection of amines into virgin females did not influence sexual receptivity, OA caused a decrease in oviposition during the 24 h following injection. Topical application of the JH analog methoprene to virgins caused an increase in DA, and a decline in mating propensity, but did not influence other amines or the oviposition rate. The results suggest the decline in OA observed immediately after mating may promote egg laying, and that male-derived JH may induce an increase in DA that could account for the post-mating loss of sexual receptivity.
交配会引起牧草盲蝽（Lygus hesperus Knight，半翅目：盲蝽科）行为和生理上的变化。在接受了包括系统调节剂保幼激素（JH）在内的精液产物后，雌性进入持续数天的交配后时期，在此期间它们提高产卵率并对再次交配失去兴趣。为了阐明牧草盲蝽这些行为变化的调控机制，在交配后5分钟、1小时和24小时对雌性头部的生物胺进行定量，并使用高效液相色谱结合电化学检测将其与未交配雌性的水平进行比较。交配后1小时和24小时多巴胺（DA）显著增加，5分钟和1小时后章鱼胺（OA）减少。血清素不会因交配而改变，但酪胺在5分钟后显著降低。虽然向未交配雌性注射胺类不会影响性接受能力，但OA在注射后的24小时内会导致产卵减少。向未交配雌性局部施用JH类似物烯虫酯会导致DA增加，交配倾向下降，但不会影响其他胺类或产卵率。结果表明，交配后立即观察到的OA下降可能会促进产卵，并且雄性来源的JH可能会诱导DA增加，这可能解释了交配后性接受能力的丧失。
