Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Dev Comp Immunol. 2014 May;44(1):100-10. doi: 10.1016/j.dci.2013.11.017. Epub 2013 Dec 9.
Yellow head virus (YHV) is one of the most widespread viruses seriously affecting black tiger shrimp (Penaeus monodon) cultivation. A previous microarray study demonstrated that clathrin coat assembly protein 17 (AP17) was significantly up-regulated after YHV infection (Pongsomboon et al., 2011). Clathrin coat AP17 is a part of the assembly protein σ2 (AP-2) complex which is involved in clathrin-mediated endocytosis. Quantitative RT-PCR (qRT-PCR) revealed that the clathrin coat AP17 gene was up-regulated 3-fold at 12 h post YHV infection. In addition, immunofluorescence microscopy showed that clathrin coat AP17 was highly expressed in the cytoplasm of the YHV-infected hemocytes. Knockdown of the clathrin coat AP17 gene dramatically reduced YHV replicativity by 32-fold. Interestingly, shrimp pre-treated with chlorpromazine, a commercial drug that inhibits clathrin-dependent endocytosis, exhibited significantly low levels of YHV infection. Taken together, these results suggest that clathrin-mediated endocytosis is involved in YHV propagation in P. monodon.
黄头病毒（YHV）是一种严重影响养殖斑节对虾（Penaeus monodon）的最广泛的病毒之一。先前的微阵列研究表明，YHV 感染后网格蛋白外套组装蛋白 17（AP17）显著上调（Pongsomboon 等人，2011 年）。网格蛋白外套 AP17 是参与网格蛋白介导的胞吞作用的组装蛋白 σ2（AP-2）复合物的一部分。定量 RT-PCR（qRT-PCR）显示，YHV 感染后 12 小时网格蛋白外套 AP17 基因上调 3 倍。此外，免疫荧光显微镜显示，YHV 感染的血细胞中细胞质中高度表达网格蛋白外套 AP17。网格蛋白外套 AP17 基因的敲低使 YHV 的复制能力降低了 32 倍。有趣的是，用氯丙嗪预处理的虾，一种抑制网格蛋白依赖性胞吞作用的商业药物，表现出 YHV 感染的水平显著降低。综上所述，这些结果表明，网格蛋白介导的胞吞作用参与了 P. monodon 中的 YHV 传播。
