Pinyaphong Porntippa, La-Up Aroon
Department of Chemistry, Faculty of Science and Technology, Uttaradit Rajabhat University, Uttaradit, 53000, Thailand.
Mahidol University, Nakhonsawan Campus, Nakhonsawan, 60130, Thailand.
Heliyon. 2024 Jul 26;10(15):e35349. doi: 10.1016/j.heliyon.2024.e35349. eCollection 2024 Aug 15.
This study investigates the application of crude glycerol to the production of 1,3-propanediol by immobilized cells of . This is a novel application of a naturally occurring producer obtained from a wastewater storage pond in Thailand. Crude glycerol was obtained through the methanolysis of palm oil, which was catalyzed using rice bran lipase. Ten components of the fermentation medium were screened using a Plackett-Burman design. The statistical significance of the results was determined using multiple linear regression with a backward elimination approach. The significance level was set to 5 % ( < 0.05). Only crude glycerol, (NH)SO, MgSO, and CaCl significantly affected 1,3-propanediol production by immobilized . Furthermore, preliminary screenings of environmental conditions used for 1,3-propanediol production were conducted using a Plackett-Burman design. The results showed that the temperature, time, and quantity of immobilized cells were factors that significantly affected 1,3-propanediol yield. Therefore, the quantities of crude glycerol, (NH)SO, MgSO, and CaCl and the temperature, time, and quantity of immobilized cells were optimized using response surface methodology based on a Box-Behnken design. The model predicted a maximum 1,3-propanediol yield of 45.68 g/L with the following conditions: 60 g/L crude glycerol, 5 g/L (NH)SO, 0.55 g/L MgSO, 0.05 g/L CaCl, a fermentation duration of 101 h, and a temperature of 25 °C, with 250 g of immobilized cells. The validation trials confirmed a production level of 44.12 ± 1.81 g/L, indicating a 2.86-fold production increase relative to the control group. Overall, this study demonstrates the potential of using crude glycerol as a substrate to improve the yields of 1,3-propanediol produced by .
本研究考察了粗甘油在由固定化细胞生产1,3 - 丙二醇中的应用。这是对从泰国一个废水储存池获得的天然生产者的一种新应用。粗甘油通过米糠脂肪酶催化的棕榈油甲醇解获得。使用Plackett - Burman设计筛选了发酵培养基的十种成分。结果的统计学显著性通过采用向后消除法的多元线性回归来确定。显著性水平设定为5%（P < 0.05）。只有粗甘油、(NH₄)₂SO₄、MgSO₄和CaCl显著影响固定化细胞生产1,3 - 丙二醇。此外，使用Plackett - Burman设计对用于生产1,3 - 丙二醇的环境条件进行了初步筛选。结果表明，温度、时间和固定化细胞数量是显著影响1,3 - 丙二醇产量的因素。因此，基于Box - Behnken设计，使用响应面法对粗甘油、(NH₄)₂SO₄、MgSO₄和CaCl的用量以及温度、时间和固定化细胞数量进行了优化。该模型预测在以下条件下1,3 - 丙二醇的最大产量为45.68 g/L：60 g/L粗甘油、5 g/L (NH₄)₂SO₄、0.55 g/L MgSO₄、0.05 g/L CaCl、发酵持续时间101 h、温度25°C以及250 g固定化细胞。验证试验证实产量水平为44.12 ± 1.81 g/L，表明相对于对照组产量提高了2.86倍。总体而言，本研究证明了使用粗甘油作为底物提高由[未提及具体微生物名称]生产的1,3 - 丙二醇产量的潜力。
