Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, Espoo, Finland.
Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, Espoo, Finland.
Bioresour Technol. 2014 Feb;153:15-22. doi: 10.1016/j.biortech.2013.11.060. Epub 2013 Dec 1.
In the enzymatic hydrolysis of lignocellulose materials, the recycling of the solid residue has previously been considered within the context of enzyme recycling. In this study, a steady state investigation of a solids-recycling process was made with pretreated wheat straw and compared to sequential and batch hydrolysis at constant reaction times, substrate feed and liquid and enzyme consumption. Compared to batch hydrolysis, the recycling and sequential processes showed roughly equal hydrolysis yields, while the volumetric productivity was significantly increased. In the 72h process the improvement was 90% due to an increased reaction consistency, while the solids feed was 16% of the total process constituents. The improvement resulted primarily from product removal, which was equally efficient in solids-recycling and sequential hydrolysis processes. No evidence of accumulation of enzymes beyond the accumulation of the substrate was found in recycling. A mathematical model of solids-recycling was constructed, based on a geometrical series.
在木质纤维素材料的酶解中，固体残渣的回收以前被认为是酶回收的一部分。在这项研究中，用预处理的麦草进行了固体制剂循环的稳态研究，并与恒反应时间、底物进料和液体及酶消耗的连续和分批水解进行了比较。与分批水解相比，回收和连续过程的水解产率大致相等，而体积生产率显著提高。在 72h 的过程中，由于反应一致性的提高，改进了 90%，而固体制剂的进料仅为总过程成分的 16%。改进主要来自于产物的去除，在固体制剂循环和连续水解过程中，产物的去除效率是相等的。在回收过程中，没有发现酶的积累超过底物的积累。基于几何级数，构建了固体制剂循环的数学模型。
