Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
Bioresour Technol. 2014 Sep;167:41-5. doi: 10.1016/j.biortech.2014.05.034. Epub 2014 May 20.
Fed-batch enzymatic hydrolysis process from alkali-pretreated sugarcane bagasse was investigated to increase solids loading, produce high-concentration fermentable sugar and finally to reduce the cost of the production process. The optimal initial solids loading, feeding time and quantities were examined. The hydrolysis system was initiated with 12% (w/v) solids loading in flasks, where 7% fresh solids were fed consecutively at 6h, 12h, 24h to get a final solids loading of 33%. All the requested cellulase loading (10 FPU/g substrate) was added completely at the beginning of hydrolysis reaction. After 120 h of hydrolysis, the maximal concentrations of cellobiose, glucose and xylose obtained were 9.376 g/L, 129.50 g/L, 56.03 g/L, respectively. The final total glucan conversion rate attained to 60% from this fed-batch process.
从碱预处理甘蔗渣的分批补料酶解过程入手，提高固含率，生产高浓度可发酵糖，最终降低生产成本。考察了最佳初始固含率、补料时间和补料量。水解体系在摇瓶中以 12%（w/v）的固含率启动，在 6h、12h、24h 时连续补加 7%的新鲜固含率，最终固含率达到 33%。所有所需纤维素酶用量（10FPU/g 底物）均在水解反应开始时一次性加入。水解 120 h 后，获得的纤维二糖、葡萄糖和木糖的最高浓度分别为 9.376 g/L、129.50 g/L、56.03 g/L。通过该分批补料过程，最终总葡聚糖转化率达到 60%。
