Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica-RJ, Brazil.
Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, 23890-000, Seropédica-RJ, Brazil.
Exp Parasitol. 2014 Feb;137:41-5. doi: 10.1016/j.exppara.2013.12.003. Epub 2013 Dec 11.
The aims of this work were to identify the compounds and to investigate the acaricidal activity of the essential oil of Lippia sidoides for unengorged larvae and nymphs of Rhipicephalus sanguineus and Amblyomma cajennense. The oil was analyzed by gas chromatography and gas chromatography/mass spectrometry. In total, 22 compounds comprising 98.5% of the total peak area were identified. The major constituent of the essential oil was thymol (69.9%). The acaricidal activity against larvae and nymphs was assessed using a modified larval packet test. In all experiments, oils were tested at concentrations of 2.35, 4.70, 9.40 14.10 and 18.80 mg/mL. The mortalities of larvae and nymphs of R. sanguineus were 20.6, 47.8, 73.6, 99.5 and 99.0% and 12.0, 50.0, 76.3, 96.0 and 96.1%, respectively. For larvae and nymphs of A. cajennense the rates of mortality were 41.9, 63.3, 77.8, 82.5 and 100.0% and 0.0, 32.8, 64.8, 71.1 and 94.0%, respectively. The LC 90 values of the L. sidoides oil were 11.56 and 12.97 mg/mL for larvae and nymphs of R. sanguineus and 15.70 and 18.52 mg/mL for larvae and nymphs of A. cajennense, respectively. The essential oil from L. sidoides has acaricidal activity on unengorged larvae and nymphs of R. sanguineus and A. cajennense.
本研究旨在鉴定紫茎泽兰精油的成分，并研究其对未饱血的曼氏硬蜱幼虫和若虫以及草原革蜱幼虫和若虫的杀蜱活性。采用气相色谱和气相色谱/质谱联用技术对精油进行分析。共鉴定出 22 种化合物，占总峰面积的 98.5%。精油的主要成分为百里酚（69.9%）。使用改良的幼虫包被法测试了精油对幼虫和若虫的杀蜱活性。在所有实验中，精油均以 2.35、4.70、9.40、14.10 和 18.80 mg/mL 的浓度进行测试。曼氏硬蜱幼虫和若虫的死亡率分别为 20.6%、47.8%、73.6%、99.5%和 99.0%，12.0%、50.0%、76.3%、96.0%和 96.1%。草原革蜱幼虫和若虫的死亡率分别为 41.9%、63.3%、77.8%、82.5%和 100.0%，0.0%、32.8%、64.8%、71.1%和 94.0%。L. sidoides 精油对 R. sanguineus 幼虫和若虫的 LC90 值分别为 11.56 和 12.97 mg/mL，对 A. cajennense 幼虫和若虫的 LC90 值分别为 15.70 和 18.52 mg/mL。紫茎泽兰精油对未饱血的曼氏硬蜱幼虫和若虫以及草原革蜱幼虫和若虫均具有杀蜱活性。
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