Hoffmann G E, Schmidt D, Bastian B, Guder W G
J Clin Chem Clin Biochem. 1986 Nov;24(11):871-5. doi: 10.1515/cclm.1986.24.11.871.
A photometric method for the determination of phospholipase A is described, in which fatty acids are liberated from phosphatidylcholine and measured by a discontinuous enzymatic test. Optimal test conditions were evaluated using purified phospholipase A from human and porcine pancreas. The enzyme activities measured in normal human sera are in the range of 0 to 10 U/l. Pathological values reach more than 100 U/l. There is no correlation with the activities of lipase or amylase in cases of acute pancreatitis.
本文描述了一种测定磷脂酶A的光度法，该方法中脂肪酸从磷脂酰胆碱中释放出来，并通过不连续酶促试验进行测定。使用从人胰腺和猪胰腺中纯化得到的磷脂酶A评估了最佳试验条件。在正常人血清中测得的酶活性范围为0至10 U/l。病理值超过100 U/l。在急性胰腺炎病例中，该酶活性与脂肪酶或淀粉酶的活性无相关性。
