Fletcher J N, Zak K, Virji M, Heckels J E
J Gen Microbiol. 1986 Jun;132(6):1611-20. doi: 10.1099/00221287-132-6-1611.
Hybrid cell lines have been derived which produce monoclonal antibodies reacting with outer membrane protein I from Neisseria gonorrhoeae strain P9. The antibodies obtained showed variable reactivity with other strains but one antibody recognized an epitope present on all of the strains tested which expressed the protease sensitive protein IB. Purified IgG labelled with 125I was used in competitive radioimmunoassays with unlabelled antibody to investigate the spacial distribution of the epitopes recognized. Each pair of antibodies showed some degree of inhibition. The relative magnitude of inhibition suggested that the conserved epitope lies within a variable region containing other epitopes which determine the antigenic specificity of the protein. Western blotting of peptides derived by proteolytic digestion of protein IB revealed that the conserved epitope is located close to the chymotrypsin cleavage site within a 7000 Mr surface exposed region of the molecule.
已获得杂交细胞系，其产生与淋病奈瑟菌P9株外膜蛋白I发生反应的单克隆抗体。所获得的抗体与其他菌株显示出不同的反应性，但有一种抗体识别所有测试菌株上存在的一个表位，这些菌株表达蛋白酶敏感蛋白IB。用125I标记的纯化IgG与未标记抗体进行竞争性放射免疫测定，以研究所识别表位的空间分布。每对抗体都显示出一定程度的抑制作用。抑制作用的相对大小表明，保守表位位于一个可变区内，该可变区包含其他决定该蛋白抗原特异性的表位。对蛋白IB进行蛋白水解消化得到的肽进行蛋白质印迹分析表明，保守表位位于该分子Mr 7000表面暴露区域内靠近胰凝乳蛋白酶切割位点的位置。
