Department of Ophthalmology, Medical University Innsbruck, Innsbruck, Austria.
Invest Ophthalmol Vis Sci. 2014 Jan 20;55(1):337-44. doi: 10.1167/iovs.13-12978.
To determine the plasma levels of platelet-derived growth factor-B (PDGF-B), VEGF, and TNF-α in patients with neovascular AMD and in patients with diabetic macular edema (DME).
Thirty patients with neovascular AMD, 30 patients with DME, and 12 healthy controls were included in this prospective study. The concentrations of PDGF-B, VEGF, and TNF-α were measured by ELISA.
The PDGF-B concentration in the plasma of controls was (median [25th-75th percentile]) 263.5 (162.0-513.3) pg/mL and in patients with DME 219.0 (122.8-604.8) pg/mL. In patients with neovascular AMD, PDGF-B levels were significantly higher with a median plasma concentration of 783.5 (289.3-1183.5) pg/mL (P = 0.003). The VEGF concentrations in patients with DME 33.0 (21.8-73.0) pg/mL and in patients with neovascular AMD 55.0 (37.0-116.3) pg/mL showed no significant differences (P = 0.159). A positive correlation of PDGF-B and VEGF plasma levels was found in patients with neovascular AMD and in patients with DME (r = 0.683, P < 0.001, and r = 0.612, P < 0.001, respectively). No significant differences of systemic TNF-α levels could be found between the three study groups.
Patients with neovascular AMD have significantly higher plasma PDGF-B levels compared with patients with DME and healthy controls. Our study data indicate that PDGF-B may be involved in the pathogenesis of neovascular AMD. (https://eudract.ema.europa.eu number, EudraCT 2010-024654-11)
测定新生血管性年龄相关性黄斑变性（AMD）患者和糖尿病性黄斑水肿（DME）患者的血小板衍生生长因子-B（PDGF-B）、血管内皮生长因子（VEGF）和肿瘤坏死因子-α（TNF-α）的血浆水平。
本前瞻性研究纳入了 30 例新生血管性 AMD 患者、30 例 DME 患者和 12 名健康对照者。通过 ELISA 法测定 PDGF-B、VEGF 和 TNF-α的浓度。
对照组血浆中 PDGF-B 的浓度（中位数[25%分位数-75%分位数]）为 263.5（162.0-513.3）pg/mL，DME 患者为 219.0（122.8-604.8）pg/mL。新生血管性 AMD 患者的 PDGF-B 水平显著升高，中位数血浆浓度为 783.5（289.3-1183.5）pg/mL（P=0.003）。DME 患者的 VEGF 浓度为 33.0（21.8-73.0）pg/mL，新生血管性 AMD 患者为 55.0（37.0-116.3）pg/mL，差异无统计学意义（P=0.159）。在新生血管性 AMD 患者和 DME 患者中，PDGF-B 和 VEGF 血浆水平呈正相关（r=0.683，P<0.001；r=0.612，P<0.001）。三组研究对象的全身 TNF-α 水平无显著差异。
与 DME 患者和健康对照者相比，新生血管性 AMD 患者的血浆 PDGF-B 水平显著升高。本研究数据表明，PDGF-B 可能参与了新生血管性 AMD 的发病机制。（https://eudract.ema.europa.eu number，EudraCT 2010-024654-11）
