Ding Jing, Ge Yanqing, Zhu Baocun
School of Chemical Engineering, Taishan Medical University.
Anal Sci. 2013;29(12):1171-5. doi: 10.2116/analsci.29.1171.
We designed and synthesized a highly selective and sensitive fluorescent probe for hydrogen sulfide (H2S) based on the one step nucleophilic addition reaction between H2S and electron-poor C=C double bond. Our proposed probe displayed high selectivity for H2S over other analytes including cysteine (Cys) and glutathione (GSH), which might be because the steric hindrance of H2S is less than other thiols. Additionally, a linear relationship between fluorescence intensity and the concentrations of Na2S (0-450 μM) was obtained in an aqueous buffer solution (pH 7.4, 20 mM PBS). Particularly, we found that the adoption of the acetyl benzimidazole derivative as a recognition receptor to distinguish H2S from other thiols and analytes provides a promising methodology for the design of fluorescent probes for the determination of H2S.
我们基于硫化氢（H₂S）与缺电子碳碳双键之间的一步亲核加成反应，设计并合成了一种对硫化氢具有高选择性和高灵敏度的荧光探针。我们提出的探针相对于包括半胱氨酸（Cys）和谷胱甘肽（GSH）在内的其他分析物，对H₂S表现出高选择性，这可能是因为H₂S的空间位阻小于其他硫醇。此外，在pH 7.4、20 mM PBS的水性缓冲溶液中，荧光强度与Na₂S浓度（0 - 450 μM）之间获得了线性关系。特别地，我们发现采用乙酰苯并咪唑衍生物作为识别受体来区分H₂S与其他硫醇和分析物，为设计用于测定H₂S的荧光探针提供了一种有前景的方法。
