Hyperbaric Medical Center, University Hospital of Medicine, Tokyo Medical and Dental University, Tokyo; Japan;
J Appl Physiol (1985). 2014 Jan 15;116(2):149-55. doi: 10.1152/japplphysiol.00235.2013. Epub 2013 Dec 12.
Recently, the use of hyperbaric oxygen (HBO) treatments by elite athletes to accelerate recovery from muscle injuries has become increasingly popular. However, the mechanism of promoting muscle regeneration under HBO conditions has not yet been defined. In this study, we investigated whether HBO treatments promoted muscle regeneration and modulated muscle regulatory factor expression in a rat skeletal muscle injury model. Muscle injury was induced by injecting cardiotoxin (CTX) into the tibialis anterior (TA) muscles. As the HBO treatment, rats were placed in an animal chamber with 100% oxygen under 2.5 atmospheres absolute for 2 h/day, 5 days/wk for 2 wk. We then performed histological analyses, measured the maximum force-producing capacity of the regenerating muscle fibers, and performed quantitative RT-PCR analysis of muscle regulatory factor mRNAs. The cross-sectional areas and maximum force-producing capacity of the regenerating muscle fibers were increased by HBO treatment after injury. The mRNA expression of MyoD, myogenin, and IGF-1 increased significantly in the HBO group at 3 and 5 days after injury. The number of Pax7(+)/MyoD(+), Pax7(-)/MyoD(+), and Pax7(+)/BrdU(+)-positive nuclei was increased by HBO treatment. In this study, we demonstrated that HBO treatment accelerated satellite cell proliferation and myofiber maturation in rat muscle that was injured by a CTX injection. These results suggest that HBO treatment accelerates healing and functional recovery after muscle injury.
最近，高压氧（HBO）治疗在精英运动员中越来越受欢迎，用于加速肌肉损伤的恢复。然而，HBO 条件下促进肌肉再生的机制尚未确定。在这项研究中，我们研究了 HBO 治疗是否在大鼠骨骼肌损伤模型中促进肌肉再生和调节肌肉调节因子表达。通过向胫骨前肌（TA）肌肉注射心脏毒素（CTX）诱导肌肉损伤。作为 HBO 治疗，大鼠每天在 2.5 个大气压的 100%氧气动物室内放置 2 小时，每周 5 天，持续 2 周。然后我们进行组织学分析，测量再生肌纤维的最大产力能力，并进行肌肉调节因子 mRNA 的定量 RT-PCR 分析。损伤后 HBO 治疗增加了再生肌纤维的横截面积和最大产力能力。HBO 组在损伤后 3 天和 5 天，MyoD、myogenin 和 IGF-1 的 mRNA 表达显著增加。HBO 治疗增加了 Pax7(+)/MyoD(+), Pax7(-)/MyoD(+), 和 Pax7(+)/BrdU(+)阳性核的数量。在这项研究中，我们证明了 HBO 治疗加速了 CTX 注射损伤大鼠肌肉中卫星细胞的增殖和肌纤维的成熟。这些结果表明，HBO 治疗加速了肌肉损伤后的愈合和功能恢复。
