Ponnappan U, Cinader B, Gerber V, Blaser K
Scand J Immunol. 1987 Jan;25(1):45-54. doi: 10.1111/j.1365-3083.1987.tb01045.x.
Age-related changes in antibody response and tolerance inducibility are polymorphic; in this paper isotype changes in ageing C57BL/6 mice are examined. Female C57BL/6 mice of various ages were immunized with either heat-aggregated RGG (a-RGG) or phosphorylcholine conjugate of RGG (PC-RGG); other animals of the same ages were given aggregate free RGG, followed by injections with either aggregated RGG or haptenated RGG. Sera from these four groups were analysed for antibody isotype. The data presented here indicate that age-related changes in isotype predominance and magnitude are different for different determinants. The capacity to be down-regulated appeared to undergo different age-related changes with different isotypes: there is split tolerance in isotypes. Age-dependent changes in T- and B-cell tolerance could be deduced by comparing responses of animals to hapten and to carrier determinants. In 5-week-old animals tolerance to hapten was more profound than tolerance to carrier. It was concluded that T-cell regulation dominated the response at this age. With advancing age, i.e. by 95 weeks, tolerance is observed in response to hapten but not in response to carrier determinants. We concluded that suppressor cells were induced by aggregate free RGG and affected the response of 'naive' but not of 'experienced' B cells.
抗体应答和耐受诱导能力的年龄相关变化具有多态性；本文研究了衰老的C57BL/6小鼠的抗体亚型变化。用热聚集的RGG（a-RGG）或RGG的磷酸胆碱缀合物（PC-RGG）免疫不同年龄的雌性C57BL/6小鼠；给相同年龄的其他动物注射无聚集物的RGG，随后注射聚集的RGG或半抗原化的RGG。分析这四组动物血清中的抗体亚型。此处呈现的数据表明，不同决定簇的亚型优势和强度的年龄相关变化有所不同。下调能力似乎随不同的抗体亚型经历不同的年龄相关变化：存在抗体亚型的分裂耐受。通过比较动物对半抗原和载体决定簇的应答，可以推断出T细胞和B细胞耐受的年龄依赖性变化。在5周龄的动物中，对半抗原的耐受比对载体的耐受更明显。得出的结论是，在这个年龄段T细胞调节主导了应答。随着年龄的增长，即到95周时，对半抗原的应答出现耐受，但对载体决定簇的应答未出现耐受。我们得出结论，无聚集物的RGG诱导了抑制细胞，并且抑制细胞影响“未接触过抗原的”B细胞而非“接触过抗原的”B细胞的应答。
