Universiti Sains Malaysia, School of Dental Sciences, Health Campus, Orthodontic Unit, Kota Bharu, Kelantan 16150, MalaysiaeAlnahrain University, Baghdad, Iraq.
Universiti Sains Malaysia, School of Dental Sciences, Health Campus, Conservative Department, Kota Bharu, Kelantan 16150, Malaysia.
J Biomed Opt. 2013 Dec;18(12):128001. doi: 10.1117/1.JBO.18.12.128001.
Bone regeneration is essential in medical treatment, such as in surgical bone healing and orthodontics. The aim of this study is to examine the effect of different powers of 940 nm diode low-level laser treatment (LLLT) on osteoblast cells during their proliferation and differentiation stages. A human fetal osteoblast cell line was cultured and treated with LLLT. The cells were divided into experimental groups according to the power delivered and periods of exposure per day for each laser power. The (3-(4,5-dimethylthiazol-2yl)-2,5 diphenyl tetrazolium bromide) (MTT) assay was used to determine cell proliferation. Both alkaline phosphatase and osteocalcin activity assays were assessed for cell differentiation. All treatment groups showed a significant increase in cell proliferation and differentiation compared to the control group. Regarding the exposure time, the subgroups treated with the LLLT for 6 min showed higher proliferation and differentiation rates for the powers delivered, the 300-mW LLLT group significantly increased the amount of cell proliferation. By contrast, the 100 and 200 mW groups showed significantly greater amounts of cell differentiation. These results suggest that the use of LLLT may play an important role in stimulating osteoblast cells for improved bone formation.
骨再生在医学治疗中至关重要，如在外科骨愈合和正畸学中。本研究旨在研究不同功率的 940nm 二极管低水平激光治疗（LLLT）对成骨细胞增殖和分化阶段的影响。培养人胎成骨细胞系并进行 LLLT 处理。根据每个激光功率的传递功率和每天暴露的时间段，将细胞分为实验组。使用（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物）（MTT）测定法确定细胞增殖。碱性磷酸酶和骨钙素活性测定法用于评估细胞分化。与对照组相比，所有治疗组的细胞增殖和分化均显著增加。关于暴露时间，用 LLLT 处理 6 分钟的亚组显示出更高的增殖和分化率，300 mW LLLT 组显著增加了细胞增殖的数量。相比之下，100 和 200 mW 组显示出明显更多的细胞分化。这些结果表明，使用 LLLT 可能在刺激成骨细胞以改善骨形成方面发挥重要作用。
