Teslenko L V, Zvonarev O F, Plakhov S A, Sorkin A D, Nikol'skiĭ N N
Tsitologiia. 1986 Oct;28(10):1097-102.
Internalization process of the fluoresceinisothiocyanate-labeled alpha 2-macroglobulin (MG-F) in Swiss 3T3 cells has been studied. The presence of internalized MG-F, formation of endocytotic vesicles and endosomal pH value were estimated visually on living monolayer cells with TV microscope, on the base of supervidicon LI-702. The use of the TV method allowed to obtain a series of photographs of cell images without any significant dye fading. A brief characteristics of microprocess control system is presented. The influence of various chemical agents on MG-F internalization and endosomal pH value was investigated. It was shown that dansylcadaverine (150-200 microM) and monensin (10-20 microM) inhibited MG-F internalization. Methylamine, chloroquine and monensin quickly enhanced the endosomal pH value, while other amines did not, for example dansylcadaverine.
已对异硫氰酸荧光素标记的α2-巨球蛋白（MG-F）在瑞士3T3细胞中的内化过程进行了研究。通过基于超正析像管LI-702的电视显微镜，在活的单层细胞上直观估计内化MG-F的存在、内吞小泡的形成以及内体pH值。使用电视方法能够获得一系列细胞图像照片，且染料没有明显褪色。文中给出了微处理控制系统的简要特性。研究了各种化学试剂对MG-F内化和内体pH值的影响。结果表明，丹磺酰尸胺（150 - 200微摩尔）和莫能菌素（10 - 20微摩尔）抑制MG-F的内化。甲胺、氯喹和莫能菌素能迅速提高内体pH值，而其他胺类如丹磺酰尸胺则不能。
