Murphy R F, Powers S, Verderame M, Cantor C R, Pollack R
Cytometry. 1982 May;2(6):402-6. doi: 10.1002/cyto.990020608.
The binding of a fluorescein-isothiocyanate derivative of insulin to Swiss 3T3 cells was measured by flow cytometry. The kinetics of the subsequent internalization were also measured; at a concentration of 1 microM labeled insulin approximately 25% of the internalization was insulin-specific. The kinetics of endocytosis were contrasted to those of fluorescent derivatives of histone and dextran. In addition, the fusion of endocytic vesicles containing insulin or dextran with lysosomes was detected by measuring the pH-dependent increase in fluorescein fluorescein fluorescence caused by the addition of chloroquine. The application of these results to the analysis of growth control by insulin and related hormones is discussed.
通过流式细胞术测定胰岛素异硫氰酸荧光素衍生物与瑞士3T3细胞的结合。还测定了随后内化的动力学；在1微摩尔标记胰岛素的浓度下，约25%的内化是胰岛素特异性的。将内吞作用的动力学与组蛋白和葡聚糖荧光衍生物的动力学进行了对比。此外，通过测量加入氯喹后荧光素荧光的pH依赖性增加，检测了含有胰岛素或葡聚糖的内吞小泡与溶酶体的融合。讨论了这些结果在胰岛素及相关激素生长控制分析中的应用。
