Gao Tianshu, Shi Rui, Qi Tengche, Yin Huisi, Mei Lan, Han Xiaoqing, Cui Peng
Chronic Disease Dept. of Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, No.72 Chongshan East Rd., Shenyang, 110032, China,
Biol Trace Elem Res. 2014 Feb;157(2):130-7. doi: 10.1007/s12011-013-9873-7. Epub 2013 Dec 13.
This study aimed to compare the effect of excess iodine and herbs with excess iodine on treating iodine deficiency-induced goiter from the perspective of oxidative stress and to measure selenium values in Chinese herbs. One hundred twenty 4-week-old Wistar rats were selected and randomly divided into four groups after inducing iodine-deficiency goiter: normal control group (NC), model control group (MC), iodine excess group (IE), and herbs with iodine excess group (HIE). The activities of oxidative enzymes and levels of oxidative products were measured using biochemical tests. The expression of 4-hydroxynonenal (4-HNE) in the thyroid was detected by immunohistochemistry and the expression of peroxiredoxin 5 (PRDX5) by the Western blot and immunohistochemistry. Selenium values in iodine-excessive herbs were measured by hydride generation-atomic fluorescence spectrometry. The herbs with iodine excess were tested to contain rich selenium. The activities of superoxide dismutase (SOD) and PRDX5 increased markedly, and the values of malondialdehyde (MDA) and 4-HNE decreased significantly in the HIE group. In conclusion, compared with excess iodine, herbs with excess iodine damaged thyroid follicular cells less, which may be related to the increase of antioxidant capacity and rich selenium values in iodine-excessive herbs.
本研究旨在从氧化应激角度比较过量碘与含过量碘中药对碘缺乏所致甲状腺肿的治疗效果，并测定中药中的硒含量。选取120只4周龄Wistar大鼠，诱导碘缺乏性甲状腺肿后随机分为四组：正常对照组（NC）、模型对照组（MC）、过量碘组（IE）和含过量碘中药组（HIE）。采用生化检测方法测定氧化酶活性和氧化产物水平。通过免疫组织化学检测甲状腺中4-羟基壬烯醛（4-HNE）的表达，通过蛋白质免疫印迹法和免疫组织化学检测过氧化物酶体增殖物激活受体5（PRDX5）的表达。采用氢化物发生-原子荧光光谱法测定含过量碘中药中的硒含量。检测发现含过量碘中药富含硒。HIE组超氧化物歧化酶（SOD）和PRDX5活性显著升高，丙二醛（MDA）和4-HNE值显著降低。综上所述，与过量碘相比，含过量碘中药对甲状腺滤泡细胞的损伤较小，这可能与含过量碘中药抗氧化能力增强及硒含量丰富有关。
