Rastad J, Wilander E, Lindgren P G, Ljunghall S, Stenkvist B G, Akerström G
Acta Cytol. 1987 Jan-Feb;31(1):45-7.
A medullary carcinoma of the thyroid was preoperatively diagnosed on ultrasonically guided fine needle aspiration biopsies. After cytocentrifugation, the tumor cells displayed a dense cytoplasmic silver granulation with the Sevier-Munger technique when applied to air-dried or acetone-ethanol-fixed samples and an obvious calcitonin immunoreactivity after fixation in Bouin's fluid. These methods may prove useful in the identification of nonpalpable metastases and recurrences of medullary carcinomas of the thyroid, especially since the cytologic typing of medullary thyroid carcinoma cells may be difficult with routine stainings.
甲状腺髓样癌经超声引导下细针穿刺活检术前确诊。细胞离心后，应用Sevier-Munger技术，在空气干燥或丙酮-乙醇固定的样本上，肿瘤细胞显示出浓密的细胞质银颗粒，在Bouin液固定后显示出明显的降钙素免疫反应性。这些方法可能有助于识别甲状腺髓样癌不可触及的转移灶和复发灶，特别是因为常规染色对甲状腺髓样癌细胞进行细胞学分型可能存在困难。
