Dziubakiewicz Ewelina, Buszewski Bogusław
Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Toruń, Poland.
Electrophoresis. 2014 Apr;35(8):1160-4. doi: 10.1002/elps.201300588. Epub 2014 Jan 28.
Uncontrolled aggregation of bacterial cells is a significant disadvantage of electrophoretic separations. Various aspects of the electrophoretic behavior of different strains of Gram-positive Bacillus cereus, Bacillus subtilis, Sarcina lutea, Staphylococcus aureus(1), and Micrococcus luteus bacteria and Gram-negative Escherichia coli bacteria were investigated in this study. Our findings indicate that bacteria can be rapidly analyzed by CZE with surface charge modification by calcium ions (Ca(2+)). Bound Ca(2+) ions increase zeta potential to more than 2.0 mV and significantly reduce repulsive forces. Under the above conditions, bacterial cells create compact aggregates, and fewer high-intensity signals are observed in electropherograms. The above can be attributed to the bridging effect of Ca(2+) between bacterial cells. CE was performed to analyze bacterial aggregates in an isotachophoretic mode. A single peak was observed in the electropherogram.
细菌细胞的无控制聚集是电泳分离的一个显著缺点。本研究调查了革兰氏阳性蜡样芽孢杆菌、枯草芽孢杆菌、藤黄八叠球菌、金黄色葡萄球菌（1）和藤黄微球菌以及革兰氏阴性大肠杆菌不同菌株电泳行为的各个方面。我们的研究结果表明，通过钙离子（Ca(2+)）进行表面电荷修饰的毛细管区带电泳（CZE）可以快速分析细菌。结合的Ca(2+)离子使zeta电位增加到2.0 mV以上，并显著降低排斥力。在上述条件下，细菌细胞形成紧密的聚集体，并且在电泳图中观察到的高强度信号较少。上述情况可归因于Ca(2+)在细菌细胞之间的桥接作用。采用等速电泳模式进行毛细管电泳以分析细菌聚集体。在电泳图中观察到一个单峰。
