Baumgarten C R, Naclerio R M, Togias A G, Lichtenstein L M, Norman P S, Proud D
Adv Exp Med Biol. 1986;198 Pt B:189-92. doi: 10.1007/978-1-4757-0154-8_23.
Allergic individuals and nonallergic controls were subjected to nasal challenge with allergen; and nasal washes, obtained before and after challenge, were assayed for high molecular weight kininogen (HMWK), total kininogen (TK), albumin and kinins. Following challenge of allergic individuals, HMWK, TK, kinin and albumin all increased dramatically, correlating (p less than 0.001) with the onset of clinical symptoms and with increases in histamine and TAME-esterase activity. No such increases were seen upon challenge of nonallergics. The time course of appearance and disappearance of the kininogens, kinins and albumin were all highly correlated (p less than 0.001 in each case) by linear regression analysis, as were the increases in kinin and each of the proteins during antigen titrations. For each individual, the plasma ratio of HMWK/TK was similar to the ratio of these two proteins in post-challenge nasal washes from the same individual. These findings are consistent with the hypothesis that, during the allergic reaction, vascular permeability increases, allowing a transudation of kininogens from plasma into nasal secretions, where they can provide substrate for kinin-forming enzymes.
过敏个体和非过敏对照者接受变应原鼻腔激发试验；在激发试验前后采集鼻腔灌洗液，检测其中高分子量激肽原（HMWK）、总激肽原（TK）、白蛋白和激肽的含量。在过敏个体接受激发试验后，HMWK、TK、激肽和白蛋白均显著增加，与临床症状的出现以及组胺和TAME酯酶活性的增加相关（p<0.001）。非过敏个体接受激发试验后未出现此类增加。通过线性回归分析，激肽原、激肽和白蛋白的出现和消失时间过程均高度相关（每种情况p<0.001），抗原滴定期间激肽和每种蛋白质的增加情况也是如此。对于每个个体，血浆中HMWK/TK的比例与同一受试者激发试验后鼻腔灌洗液中这两种蛋白质的比例相似。这些发现与以下假设一致：在过敏反应期间，血管通透性增加，使得激肽原从血浆渗出到鼻腔分泌物中，在那里它们可为激肽形成酶提供底物。
