Belibasakis Georgios N, Thurnheer Thomas, Bostanci Nagihan
Oral Microbiology and Immunology, Institute of Oral Biology, Center of Dental Medicine, University of Zürich, Zürich, Switzerland.
PLoS One. 2013 Dec 10;8(12):e81581. doi: 10.1371/journal.pone.0081581. eCollection 2013.
Periodontitis is an infectious inflammatory disease that results in the destruction of the tooth-supporting (periodontal) tissues. The Gram-negative anaerobic species Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola, (also known as the "red complex" species) are highly associated with subgingival biofilms at periodontitis-affected sites. A major chemokine produced by the gingival epithelium in response to biofilm challenge, is interleukin (IL)-8. The aim of this in vitro study was to investigate the relative effect of the "red complex" species as constituents of subgingival biofilms, on the regulation of IL-8 by gingival epithelia. Multi-layered organotypic human gingival epithelial cultures were challenged with a 10-species in vitro subgingival biofilm model, or its 7-species variant, excluding the "red complex". IL-8 gene expression and secretion analyses were performed by qPCR and ELISA, respectively. After 3 h, both biofilms up-regulated IL-8 gene expression, but the presence of the "red complex" resulted in 3-fold greater response. IL-8 secretion was also up-regulated by both biofilms, with no differences between them. After 24 h, the 10-species biofilm reduced IL-8 secretion to 50% of the control, but this was not affected when the "red complex" was absent. In conclusion, as part of biofilms, "red complex" species differentially regulate IL-8 in gingival epithelia, potentially affecting the chemotactic responses of the tissue.
牙周炎是一种感染性炎症疾病，会导致牙齿支持（牙周）组织的破坏。革兰氏阴性厌氧菌牙龈卟啉单胞菌、福赛坦氏菌和齿垢密螺旋体（也被称为“红色复合体”菌种）与牙周炎患病部位的龈下生物膜高度相关。牙龈上皮细胞对生物膜刺激产生的一种主要趋化因子是白细胞介素（IL）-8。这项体外研究的目的是调查作为龈下生物膜成分的“红色复合体”菌种对牙龈上皮细胞调节IL-8的相对影响。用一种10菌种的体外龈下生物膜模型或其不含“红色复合体”的7菌种变体对多层器官型人牙龈上皮细胞培养物进行刺激。分别通过定量聚合酶链反应（qPCR）和酶联免疫吸附测定（ELISA）进行IL-8基因表达和分泌分析。3小时后，两种生物膜均上调了IL-8基因表达，但“红色复合体”的存在导致反应增强了3倍。两种生物膜也都上调了IL-8分泌，且它们之间没有差异。24小时后，10菌种生物膜将IL-8分泌降至对照的50%，但在没有“红色复合体”时这一情况未受影响。总之，作为生物膜的一部分，“红色复合体”菌种对牙龈上皮细胞中IL-8的调节存在差异，可能会影响组织的趋化反应。
