促甲状腺激素和环磷酸腺苷对激素缺乏的FRTL-5甲状腺细胞中甲状腺球蛋白基因的激活作用

Thyroglobulin gene activation by thyrotropin and cAMP in hormonally depleted FRTL-5 thyroid cells.

作者信息

Bone E, Kohn L D, Chomczynski P

出版信息

Biochem Biophys Res Commun. 1986 Dec 30;141(3):1261-6. doi: 10.1016/s0006-291x(86)80181-4.

Abstract

Thyroglobulin gene control by cAMP and thyrotropin has been compared in FRTL-5 thyroid cells maintained in the absence of serum, insulin, cortisol, and thyrotropin for 7 days. Readdition of thyrotropin induces an increase in the expression of the thyroglobulin gene with little effect on total RNA synthesis. In contrast, addition of 8-bromo cyclic AMP and cholera toxin stimulate total RNA synthesis as well as thyroglobulin gene expression. The same non-selective effect of cyclic AMP on the thyroglobulin gene was observed when transcriptional activity was measured in permeabilized FRTL-5 cells.

摘要

在无血清、胰岛素、皮质醇和促甲状腺激素的条件下培养7天的FRTL-5甲状腺细胞中，对环磷酸腺苷（cAMP）和促甲状腺激素对甲状腺球蛋白基因的调控进行了比较。重新添加促甲状腺激素会诱导甲状腺球蛋白基因表达增加，而对总RNA合成影响不大。相反，添加8-溴环磷酸腺苷和霍乱毒素会刺激总RNA合成以及甲状腺球蛋白基因表达。当在透化的FRTL-5细胞中测量转录活性时，观察到环磷酸腺苷对甲状腺球蛋白基因具有相同的非选择性作用。