Human thymocyte differentiation: an ultrastructural study using monoclonal antibodies and immunogold staining method.

The ultrastructural features of normal human thymocytes were analyzed at various stages of immunologic differentiation as defined by a panel of OKT monoclonal antibodies visualized by colloidal gold labelled antimouse IgG. The study shows that first stage thymocytes (OKT10+, OKT9+) have a typical morphology not found in more mature stages and describes the critical ultrastructural change which they undergo in becoming second stage thymocytes (OKT10+, OKT6+, OKT8+, OKT4+). No change in thymocyte fine morphology has been found in the progression from second to third stage (OKT10+, OKT3+, OKT8+ or OKT10+, OKT3+, OKT4+). Cells with ultrastructural features intermediate between those of the first and second stage have been found to react with monoclonal antibodies characterizing the first and the second stage. Ultrastructural similarity and homogeneity found in OKT8+ and OKT4+ thymic subpopulations suggests the acquisition by T lymphocytes of morphologic structures correlate with immunologic features previously found in peripheral blood T cell subpopulations to be a post-thymic process.