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正常淋巴细胞亚群的精细结构——一项使用单克隆抗体和免疫金技术的研究

The fine structure of normal lymphocyte subpopulations--a study with monoclonal antibodies and the immunogold technique.

作者信息

Matutes E, Catovsky D

出版信息

Clin Exp Immunol. 1982 Nov;50(2):416-25.

PMID:6185261
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1536696/
Abstract

The ultrastructural characteristics of normal lymphocyte subpopulations, identified by monoclonal antibodies and visualized by a colloidal gold labelled anti-mouse IgG were analysed. Our study demonstrates: (1) the major T lymphocyte subsets (OKT4+ and OKT8+) have distinct ultrastructural morphology. The majority of OKT4+ cells have a high nuclear/cytoplasmic ratio (N/C) and few cytoplasmic organelles whilst most OKT8+ cells have a low N/C ratio and numerous organelles, namely a well developed Golgi apparatus, lysosomal granules and parallel tubular arrays (PTA); (2) a unique subtype with irregular nuclear outline that resembles Sézary cells was seen in 5-10% of OKT4+ lymphocytes; (3) OKM1, a reagent that reacts with monocytes and granulocytes, is positive in a small lymphocyte subset which appears to be negative with the OKT reagents and is morphologically identical to OKT8+ cells; (4) 'hand-mirror' cells were only seen labelled with OKT8 and OKM1; (5) B lymphocytes labelled with FMC4 (anti-IA) could be distinguished from OKT3+ lymphocytes by having numerous profiles of endoplasmic reticulum (ER) and ribosomes; these were particularly prominent in lymphoplasmacytoid cells. Morphological similarities between normal T lymphocyte subsets and T neoplasias of the same membrane phenotype suggest that these disorders arise from specific T cell types present in normal peripheral blood or from common precursors.

摘要

分析了通过单克隆抗体鉴定并用胶体金标记的抗小鼠IgG可视化的正常淋巴细胞亚群的超微结构特征。我们的研究表明：（1）主要的T淋巴细胞亚群（OKT4 +和OKT8 +）具有不同的超微结构形态。大多数OKT4 +细胞具有高核/质比（N/C）且细胞质细胞器较少，而大多数OKT8 +细胞具有低N/C比且细胞器众多，即发达的高尔基体、溶酶体颗粒和平行管状排列（PTA）；（2）在5%-10%的OKT4 +淋巴细胞中可见一种具有不规则核轮廓且类似于Sezary细胞的独特亚型；（3）OKM1是一种与单核细胞和粒细胞反应的试剂，在一个小淋巴细胞亚群中呈阳性，该亚群似乎对OKT试剂呈阴性，且在形态上与OKT8 +细胞相同；（4）“手镜”细胞仅用OKT8和OKM1标记；（5）用FMC4（抗-IA）标记的B淋巴细胞可通过具有大量内质网（ER）和核糖体轮廓与OKT3 +淋巴细胞区分开来；这些在淋巴浆细胞样细胞中尤为突出。正常T淋巴细胞亚群与相同膜表型的T肿瘤之间的形态学相似性表明，这些疾病源于正常外周血中存在的特定T细胞类型或共同前体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/7d765586293e/clinexpimmunol00164-0186-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/fe932178ec3c/clinexpimmunol00164-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/fde3343b3969/clinexpimmunol00164-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/42ed807ed06e/clinexpimmunol00164-0183-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/2971796a351e/clinexpimmunol00164-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/cda047e1f1d6/clinexpimmunol00164-0184-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/b3741216bf0c/clinexpimmunol00164-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/b8d01476f13b/clinexpimmunol00164-0185-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/f642a63028ab/clinexpimmunol00164-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/7d765586293e/clinexpimmunol00164-0186-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/fe932178ec3c/clinexpimmunol00164-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/fde3343b3969/clinexpimmunol00164-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/42ed807ed06e/clinexpimmunol00164-0183-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/2971796a351e/clinexpimmunol00164-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/cda047e1f1d6/clinexpimmunol00164-0184-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/b3741216bf0c/clinexpimmunol00164-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/b8d01476f13b/clinexpimmunol00164-0185-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/f642a63028ab/clinexpimmunol00164-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d250/1536696/7d765586293e/clinexpimmunol00164-0186-b.jpg

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