Limborska S A, Korneev S A, Maleeva N E, Slominsky P A, Jincharadze A G, Ivanov P L, Ryskov A P
FEBS Lett. 1987 Feb 23;212(2):208-12. doi: 10.1016/0014-5793(87)81346-7.
Two clones have been selected from a human fibroblast cDNA bank. By DNA sequencing the clones were shown to contain Alu elements located near the ends of the cDNA inserts. DNA of the clones was used for Northern blot hybridization analysis of a number of poly(A)-containing RNAs from normal human tissues (brain, stomach, uterus, spleen, fibroblasts) and tumors (neurinoma, glioma, neuroblastoma, liposarcoma, adrenal cortex adenocarcinoma). All RNA samples reveal a heterodisperse distribution of Alu transcripts with discrete bands in the region of 7-12 S RNA. The majority of these small poly(A)+ Alu+ RNAs contain Alu sequences only in one (canonical) orientation with functional signals including the split promoter for RNA polymerase III.
已从人成纤维细胞cDNA文库中筛选出两个克隆。通过DNA测序表明，这些克隆在cDNA插入片段末端附近含有Alu元件。克隆的DNA用于对来自正常人组织（脑、胃、子宫、脾、成纤维细胞）和肿瘤（神经鞘瘤、胶质瘤、神经母细胞瘤、脂肪肉瘤、肾上腺皮质腺癌）的多种含多聚腺苷酸（poly(A)）的RNA进行Northern印迹杂交分析。所有RNA样品均显示Alu转录本呈异质分布，在7 - 12 S RNA区域有离散条带。这些小的多聚腺苷酸阳性（poly(A)+）、Alu阳性（Alu+）RNA中的大多数仅在一个（标准）方向上含有Alu序列，且带有包括RNA聚合酶III的分裂启动子在内的功能信号。
