[来自人成纤维细胞cDNA克隆文库的含Alu元件的序列：核苷酸序列及在各种组织中的表达]

[Sequences containing the Alu elements from a human fibroblast cDNA clone library: nucleotide sequence and expression in various tissues].

作者信息

Korneev S A, Maleeva N E, Dzhincharadze A G, Slominskiĭ P A, Ivanov P L

出版信息

Mol Biol (Mosk). 1986 Sep-Oct;20(5):1377-89.

Abstract

Alu containing cDNA clones were isolated from a human fibroblast cDNA library. The nucleotide sequences of two clones were determined. In both cases Alu repeats appeared to be situated in the regions presumably corresponding to the 3'-end of mRNA. The comparison of the unique sequences of those clones did not show any homology between them. Both cDNA clones contained large open reading frames, extending into the Alu regions. DNA of one clone was used as a probe for Northern blot-hybridization analysis of poly(A)+ cytoplasmic RNA from some normal and tumor human tissues. All RNA samples contained Alu-homologous transcripts, mainly in 7-12S fractions and only in one (canonical) orientation. This orientation contained functional regions including promotor region for RNA-polymerase III.

摘要

从人成纤维细胞cDNA文库中分离出含Alu的cDNA克隆。测定了两个克隆的核苷酸序列。在这两种情况下，Alu重复序列似乎位于可能对应于mRNA 3′末端的区域。对这些克隆的独特序列进行比较，未发现它们之间有任何同源性。两个cDNA克隆都含有延伸到Alu区域的大开放阅读框。用一个克隆的DNA作为探针，对来自一些正常和肿瘤人类组织的聚腺苷酸加尾细胞质RNA进行Northern印迹杂交分析。所有RNA样品都含有Alu同源转录本，主要在7 - 12S组分中，且只有一种（典型）方向。这种方向包含包括RNA聚合酶III启动子区域在内的功能区域。