Panousis D, Xepapadakis G, Lagoudianakis E, Karavitis G, Salemis N, Koronakis N, Patsouris E, Koronarchis D, Grosomanidis D, Chryssikos G, Ntasiou P, Kyriakidou V, Athanassiadou A M, Athanassiadou P
Breast Unit, Iaso General Hospiral, Athens, Greece.
J BUON. 2013 Oct-Dec;18(4):879-85.
The objective of this study was to examine the association of EZH2 and paxillin expression and DNA ploidy status with pathological parameters of breast cancer, aiming to correlate tumor phenotype with its malignant behavior.
EZH2 and paxillin expression and DNA ploidy were evaluated in imprint smear samples obtained from 105 breast tumors after surgical removal.
Increased expression of paxillin was associated with p53 expression (p=0.005), Ki-67 expression (p=0.018) and EZH2 expression (p<0.0001). EZH2 expression correlated with estrogen receptor (ER) and progesterone receptor (PR) status (p=0.01 and p=0.035, respectively), and expression of p53 and Ki-67 (p=0.007 and p<0.0001, respectively). Aneuploid tumors were significantly correlated with poor differentiation (p=0.000), stage of disease (p=0.000), size of the primary tumor (p=0.015), presence of nodal metastasis (p=0.001), ER status (p=0.008), cerbB2 status (p=0.012), and expression of Ki-67 (p=0.001) and EGFR (p=0.018). Multivariate analysis of ploidy results using paxillin and EZH2 expression as dependent variables revealed that aneuploid tumors were associated with disease stage and grade of differentiation, cerbB2 expression and EZH2 expression.
Our results show that aneuploid tumors, EZH2 expression and paxillin expression correlate with more aggressive phenotype of breast cancer.
本研究的目的是检测EZH2和桩蛋白的表达以及DNA倍体状态与乳腺癌病理参数之间的关联，旨在将肿瘤表型与其恶性行为相关联。
对手术切除的105例乳腺肿瘤的印片样本进行EZH2和桩蛋白表达以及DNA倍体的评估。
桩蛋白表达增加与p53表达（p = 0.005）、Ki-67表达（p = 0.018）和EZH2表达（p < 0.0001）相关。EZH2表达与雌激素受体（ER）和孕激素受体（PR）状态相关（分别为p = 0.01和p = 0.035），以及p53和Ki-67的表达（分别为p = 0.007和p < 0.0001）。非整倍体肿瘤与低分化（p = 0.000）、疾病分期（p = 0.000）、原发肿瘤大小（p = 0.015）、淋巴结转移情况（p = 0.001）、ER状态（p = 0.008）、cerbB2状态（p = 0.012）以及Ki-67（p = 0.001）和表皮生长因子受体（EGFR）（p = 0.018）的表达显著相关。以桩蛋白和EZH2表达作为因变量对倍体结果进行多变量分析显示，非整倍体肿瘤与疾病分期、分化程度、cerbB2表达和EZH2表达相关。
我们的结果表明，非整倍体肿瘤、EZH2表达和桩蛋白表达与乳腺癌更具侵袭性的表型相关。
