Heiskala M
Immunology. 1987 Feb;60(2):167-72.
Natural killer (NK) activity is inhibited by the contact of peripheral blood lymphocytes with primary monolayer cell cultures of both benign and malignant origin. In this study the effect of interferons (IFNs) on the inhibition has been analysed. Both alpha IFN- and gamma IFN-pretreated peripheral blood lymphocytes are effectively inhibited by monolayer target cells. IFN treatment of lymphocytes does not change cytotoxicity against the inhibitory target cells, although it enhances reactivity against NK-sensitive target cells. Treatment of monolayer cells with interferons significantly reduces their inhibitory capacity. However, diminished inhibition of NK activity by the IFN-treated target cells is not associated with increased lysis, probably due to their decreased sensitivity to natural killer cytotoxic factors (NKCF). In 18.5% of the cases studied, monolayer target cells induced endogenous IFN production in lymphocytes. In these cases no inhibition of the NK activity of the effector cells was seen. According to the results of this paper, IFNs have a dual effect on the NK regulatory system: they enhance the NK activity of the effector cells against NK-sensitive target cells, and they change the NK resistant target cells in a way that makes them less inhibitory to NK activity but simultaneously more resistant to the toxic factors secreted by NK cells.
自然杀伤（NK）活性会受到外周血淋巴细胞与良性和恶性来源的原代单层细胞培养物接触的抑制。在本研究中，分析了干扰素（IFN）对这种抑制作用的影响。经α干扰素和γ干扰素预处理的外周血淋巴细胞均受到单层靶细胞的有效抑制。干扰素处理淋巴细胞虽增强了其对NK敏感靶细胞的反应性，但并未改变其对抑制性靶细胞的细胞毒性。用干扰素处理单层细胞可显著降低其抑制能力。然而，经干扰素处理的靶细胞对NK活性抑制作用的减弱与裂解增加无关，这可能是由于它们对自然杀伤细胞毒性因子（NKCF）的敏感性降低所致。在18.5%的研究病例中，单层靶细胞诱导淋巴细胞产生内源性干扰素。在这些病例中，未观察到效应细胞的NK活性受到抑制。根据本文结果，干扰素对NK调节系统有双重作用：它们增强效应细胞对NK敏感靶细胞的NK活性，并且改变NK抗性靶细胞，使其对NK活性的抑制作用减弱，但同时对NK细胞分泌的毒性因子更具抗性。
