Heiskala M, Ylikorkala O, Timonen T
Nat Immun Cell Growth Regul. 1987;6(1):1-11.
Some primary and continuous cell cultures were tested for their capacity to regulate human natural killer (NK) activity. Primary cultures of endothelial cells, fetal fibroblasts, adult fibroblasts, amnion epithelial cells, renal parenchymal cells, and ovarian carcinoma cells inhibited NK activity when peripheral blood lymphocytes were preincubated on target cell monolayers for 18 h before testing the cytotoxicity against K-562. The supernatants of the inhibiting cell cultures were not suppressive. Prostaglandins or suppressive lymphocytes were not involved in the phenomenon. The binding capacity of the effector cells was not changed, suggesting that the suppressive signal was targeted at the cytolytic machinery of NK cells. The down-regulating capacity of the cell cultures weakened significantly during subculturing in vitro, and continuous cell lines were not inhibitory. The inactivation of NK cells may be one of the mechanisms by which target cells are protected from NK activity.
对一些原代细胞和连续细胞培养物调节人类自然杀伤（NK）活性的能力进行了测试。当在检测针对K-562的细胞毒性之前，将外周血淋巴细胞在靶细胞单层上预孵育18小时时，内皮细胞、胎儿成纤维细胞、成人成纤维细胞、羊膜上皮细胞、肾实质细胞和卵巢癌细胞的原代培养物抑制了NK活性。抑制性细胞培养物的上清液没有抑制作用。前列腺素或抑制性淋巴细胞与该现象无关。效应细胞的结合能力没有改变，这表明抑制信号是针对NK细胞的溶细胞机制。在体外传代培养期间，细胞培养物的下调能力显著减弱，并且连续细胞系没有抑制作用。NK细胞的失活可能是靶细胞免受NK活性影响的机制之一。
