Smeets A W, Pauwels R P, Beck J L, Geraedts J P, Debruyne F M, Laarakkers L, Feitz W F, Vooijs G P, Ramaekers F C
Int J Cancer. 1987 Mar 15;39(3):304-10. doi: 10.1002/ijc.2910390307.
Thirty-seven transitional-cell carcinomas (TCC) of the urinary bladder were analyzed by DNA flow cytometry (FCM). After labelling of the cell suspensions with antibodies to cytokeratin, the cytokeratin-positive cells and the non-epithelial cytokeratin-negative cells could be analyzed separately. After estimation of S- and G2M phase, 3/17 cases (18%) with a normal DNA index showed elevated proliferative levels, among cytokeratin-labelled suspensions only. Of these 17 cases, 14 showed chromosomal abnormalities. The remaining 20 cases were abnormal, irrespective of the technique used. Although immuno-labeling of tumor cells for cytokeratin in FCM increases the sensitivity of this method in detecting aneuploid tumors or tumors with high proliferation fractions, the discriminating power of chromosomal analysis of TCC is greater than FCM.
应用DNA流式细胞术（FCM）对37例膀胱移行细胞癌（TCC）进行分析。用细胞角蛋白抗体标记细胞悬液后，可分别分析细胞角蛋白阳性细胞和非上皮细胞角蛋白阴性细胞。在估计S期和G2M期后，仅在细胞角蛋白标记的悬液中，17例DNA指数正常的病例中有3例（18%）显示增殖水平升高。在这17例病例中，14例显示染色体异常。其余20例无论采用何种技术均为异常。尽管FCM中肿瘤细胞的细胞角蛋白免疫标记提高了该方法检测非整倍体肿瘤或高增殖分数肿瘤的敏感性，但TCC的染色体分析鉴别能力大于FCM。
