Coon J S, Schwartz D, Summers J L, Miller A W, Weinstein R S
Cancer. 1986 Apr 15;57(8):1594-601. doi: 10.1002/1097-0142(19860415)57:8<1594::aid-cncr2820570827>3.0.co;2-1.
Both cytogenetic analysis and flow cytometry (FCM) have prognostic efficacy in the analysis of transitional cell carcinoma (TCC) of urinary bladder. To correlate results of the two methods, we studied a unique group of patients whose tumors had undergone prospective conventional cytogenetic analysis. Paraffin blocks of these tumors were processed for FMC, then analyzed for nuclear DNA content. Of the 34 tumors processed, 30 (88%) yielded interpretable DNA histograms. In 26 (87%) of these, there was good correlation between the two methods with respect to the presence or absence of a hyperdiploid cell line. Discrepancies may have resulted from sampling error or from interpretation of a tetraploid peak as a prominent G2M region. Retrospective FCM analysis of paraffinized TCC tissue correlates well with conventional, prospective cytogenetic analysis and is applicable to the majority of urinary bladder transitional cell carcinomas.
细胞遗传学分析和流式细胞术（FCM）在膀胱移行细胞癌（TCC）分析中均具有预后评估功效。为了关联这两种方法的结果，我们研究了一组独特的患者，其肿瘤已进行前瞻性常规细胞遗传学分析。对这些肿瘤的石蜡块进行处理以用于FCM检测，然后分析核DNA含量。在处理的34个肿瘤中，30个（88%）产生了可解释的DNA直方图。其中26个（87%）在超二倍体细胞系的有无方面，两种方法之间具有良好的相关性。差异可能是由于抽样误差或将四倍体峰解释为突出的G2M区域所致。石蜡包埋的TCC组织的回顾性FCM分析与传统的前瞻性细胞遗传学分析相关性良好，并且适用于大多数膀胱移行细胞癌。
