Lin-Chao S, Bremer H
J Bacteriol. 1987 Mar;169(3):1217-22. doi: 10.1128/jb.169.3.1217-1222.1987.
The synthesis rates of the replication control RNAs of plasmid pBR322, RNAI, an inhibitor of replication, and RNAII, the preprimer, have been determined by hybridizing in vivo pulse-labeled RNA to specific, single-stranded DNA probes for RNAI and RNAII. In Escherichia coli growing in glycerol minimal medium, RNAI transcripts were made at a rate of one molecule per 30 s per plasmid; RNAII was transcribed fivefold less, at a rate of one molecule per 3 min per plasmid. It is estimated that only 1 in 20 prepriming events leads to replication.
通过将体内脉冲标记的RNA与针对RNAI和RNAII的特异性单链DNA探针杂交，测定了质粒pBR322的复制控制RNA、复制抑制剂RNAI和前引物RNAII的合成速率。在甘油基本培养基中生长的大肠杆菌中，RNAI转录本的合成速率为每个质粒每30秒产生一个分子；RNAII的转录速率则低五倍，为每个质粒每3分钟产生一个分子。据估计，每20次前引物引发事件中只有1次会导致复制。
