Department of Veterans Affairs Tennessee Valley, Nashville, TN; Department of Medicine, Vanderbilt University, Nashville, TN.
Department of Veterans Affairs Tennessee Valley, Nashville, TN; Department of Medicine, Vanderbilt University, Nashville, TN; Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN.
Biotechniques. 2013 Dec;55(6):310-2. doi: 10.2144/000114115.
Current protocols for screening proliferative factors for β cells ex vivo are time-consuming, require cell lines or dissociated islets, and often entail expensive specialized screening equipment. Here we present an efficient and lower cost alternative that utilizes intact mouse islets for the initial screening of proliferative compounds and allows confirmation of β cell proliferation from the same islets. This protocol simplifies the process, saves money, and provides a way to identify β cell proliferative factors using equipment that is ubiquitous in most laboratories.
目前用于体外筛选β细胞增殖因子的方案耗时耗力，需要细胞系或分离的胰岛，并且通常需要昂贵的专用筛选设备。在这里，我们提出了一种高效且低成本的替代方案，该方案使用完整的小鼠胰岛进行增殖化合物的初步筛选，并允许从相同的胰岛中确认β细胞增殖。该方案简化了流程，节省了资金，并提供了一种使用大多数实验室都普遍存在的设备来识别β细胞增殖因子的方法。
