一种高通量基于 PCR 的斑马鱼幼鱼尾活检基因分型方法。
A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies.
机构信息
Department of Cardiovascular Science, University of Sheffield, Medical School, Sheffield, United Kingdom.
Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield, United Kingdom.
出版信息
Biotechniques. 2013 Dec;55(6):314-6. doi: 10.2144/000114116.
Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.
在这里，我们描述了一种在受精后 72 小时（hpf）的早期对活体斑马鱼幼虫进行高通量基因分型的方法。重要的是，该技术允许从小的鳍活检中快速且经济有效地进行基于 PCR 的基因分型，而这些活检会随着胚胎的发育而再生，从而使研究人员能够选择具有所需基因型的胚胎成长为成年。
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