在48小时内对斑马鱼进行微流控辅助基因分型，存活率达100%。
Microfluidic-aided genotyping of zebrafish in the first 48 h with 100% viability.
作者信息
Samuel Raheel, Stephenson Regan, Roy Paula, Pryor Rob, Zhou Luming, Bonkowsky Joshua L, Gale Bruce K
机构信息
Department of Mechanical Engineering, University of Utah, Salt Lake City, UT, 84112, USA,
出版信息
Biomed Microdevices. 2015 Apr;17(2):43. doi: 10.1007/s10544-015-9946-9.
This paper introduces an innovative method for genotyping 1-2 days old zebrafish embryos, without sacrificing the life/health of the embryos. The method utilizes microfluidic technology to extract and collect a small amount of genetic material from the chorionic fluid or fin tissue of the embryo. Then, using conventional DNA extraction, PCR amplification, and high resolution melt analysis with fluorescent DNA detection techniques, the embryo is genotyped. The chorionic fluid approach was successful 78% of the time while the fin clipping method was successful 100% of the time. Chorionic fluid was shown to only contain DNA from the embryo and not from the mother. These results suggest a novel method to genotype zebrafish embryos that can facilitate high-throughput screening, while maintaining 100% viability of the embryo.
本文介绍了一种用于对1至2日龄斑马鱼胚胎进行基因分型的创新方法，且不会牺牲胚胎的生命/健康。该方法利用微流控技术从胚胎的绒毛膜液或鳍组织中提取并收集少量遗传物质。然后，通过常规的DNA提取、PCR扩增以及使用荧光DNA检测技术的高分辨率熔解分析，对胚胎进行基因分型。绒毛膜液方法成功率为78%，而剪鳍方法成功率为100%。研究表明，绒毛膜液仅含有胚胎的DNA，而非母体的DNA。这些结果表明了一种对斑马鱼胚胎进行基因分型的新方法，该方法可促进高通量筛选，同时保持胚胎100%的存活率。
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