Yoshino J E, Mason P W, DeVries G H
J Cell Biol. 1987 Mar;104(3):655-60. doi: 10.1083/jcb.104.3.655.
Schwann cell proliferation induced by a myelin-enriched fraction was examined in vitro. Although nearly all the Schwann cells contained material that was recognized by antisera to myelin basic protein after 24 h, only 1% of the cells were synthesizing DNA. 72 h after the addition of the mitogen a maximum of 10% of the cells incorporated [3H]thymidine. If the cultures were treated with the myelin-enriched fraction for 24 h and then washed, the number of proliferating Schwann cells decreased by 75% when compared with those cells that were incubated with the mitogen continuously. When Schwann cells were labeled with [14C]thymidine followed by a pulse of [3H]thymidine 24 h later, every Schwann cell labeled with [3H]thymidine was also labeled with [14C]thymidine. Although almost every Schwann cell can metabolize the myelin membranes within 24 h of exposure, a small population of cell initially utilizes the myelin as a mitogen, and this population continues to divide only if myelin is present in the extracellular media. The percentage of the Schwann cells that initially recognize the myelin-enriched fraction as a mitogen is dependent upon the age of the animal from which the cells were prepared.
在体外检测了富含髓磷脂的组分诱导的雪旺细胞增殖。虽然几乎所有雪旺细胞在24小时后都含有能被抗髓磷脂碱性蛋白抗血清识别的物质，但只有1%的细胞在合成DNA。加入促有丝分裂原72小时后，最多10%的细胞掺入了[3H]胸腺嘧啶核苷。如果培养物用富含髓磷脂的组分处理24小时，然后洗涤，与持续用促有丝分裂原孵育的细胞相比，增殖的雪旺细胞数量减少了75%。当雪旺细胞先用[14C]胸腺嘧啶核苷标记，24小时后再用[3H]胸腺嘧啶核苷脉冲标记时，每个用[3H]胸腺嘧啶核苷标记的雪旺细胞也都被[14C]胸腺嘧啶核苷标记。虽然几乎每个雪旺细胞在接触后24小时内都能代谢髓磷脂膜，但一小部分细胞最初将髓磷脂用作促有丝分裂原，并且只有当细胞外培养基中存在髓磷脂时，这部分细胞才会继续分裂。最初将富含髓磷脂的组分识别为促有丝分裂原的雪旺细胞百分比取决于制备细胞的动物的年龄。
