Institute of Biochemistry II, Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany.
J Cell Sci. 2014 Jan 1;127(Pt 1):3-9. doi: 10.1242/jcs.140426. Epub 2013 Dec 17.
Continuous synthesis of all cellular components requires their constant turnover in order for a cell to achieve homeostasis. To this end, eukaryotic cells are endowed with two degradation pathways - the ubiquitin-proteasome system and the lysosomal pathway. The latter pathway is partly fed by autophagy, which targets intracellular material in distinct vesicles, termed autophagosomes, to the lysosome. Central to this pathway is a set of key autophagy proteins, including the ubiquitin-like modifier Atg8, that orchestrate autophagosome initiation and biogenesis. In higher eukaryotes, the Atg8 family comprises six members known as the light chain 3 (LC3) or γ-aminobutyric acid (GABA)-receptor-associated protein (GABARAP) proteins. Considerable effort during the last 15 years to decipher the molecular mechanisms that govern autophagy has significantly advanced our understanding of the functioning of this protein family. In this Cell Science at a Glance article and the accompanying poster, we present the current LC3 protein interaction network, which has been and continues to be vital for gaining insight into the regulation of autophagy.
连续合成所有细胞成分需要不断更新，以维持细胞的内稳态。为此，真核细胞具有两种降解途径 - 泛素蛋白酶体系统和溶酶体途径。后者部分由自噬提供，自噬靶向特定囊泡（称为自噬体）中的细胞内物质到溶酶体。该途径的核心是一组关键的自噬蛋白，包括泛素样修饰物 Atg8，它协调自噬体的起始和生物发生。在高等真核生物中，Atg8 家族由六个成员组成，称为轻链 3（LC3）或 γ-氨基丁酸（GABA）-受体相关蛋白（GABARAP）蛋白。在过去的 15 年中，为了解析控制自噬的分子机制，人们付出了巨大的努力，这极大地促进了我们对该蛋白家族功能的理解。在这篇 Cell Science at a Glance 文章和配套海报中，我们展示了当前的 LC3 蛋白相互作用网络，该网络对于深入了解自噬的调控一直至关重要。
