School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Chin J Nat Med. 2013 Nov;11(6):699-704. doi: 10.1016/S1875-5364(13)60082-5.
To provide a comprehensive procedure to evaluate the contribution of the floral parts to the yield of the major components from the flowers of Trollius chinensis, to underlay the selective breeding, cultivation, development, and utilization of the flowers.
Five floral parts from eleven batches of the flowers of T. chinensis were examined by HPLC analysis for the content of orientin and vitexin, and by gravimetric analysis for their respective mass fraction. The contribution of each floral part was calculated using mathematical methods based on the results of the content and mass fraction. Variance analysis was carried out by Kruskal-Wallis H test and PCA method.
The calculated mean contributions of calyx, corolla, stamens and pistils, stalk, and ovary to the yield of both orientin and vitexin were 76.99% and 71.93%, 9.60% and 8.33%, 9.21% and 8.10%, 2.17% and 6.62%, and 2.03% and 5.02%, respectively.
The floral parts contribute unequally to the yield of orientin and vitexin, and the calyx contributes the highest and makes a significant difference compared with any other part.
提供一种全面的程序来评估金莲花花朵各部分对主要成分产量的贡献，为金莲花花朵的选择性培育、栽培、开发和利用奠定基础。
采用 HPLC 分析和重量分析两种方法，对 11 批金莲花的 5 个花朵部分进行了研究，检测了木樨草苷和荭草苷的含量，以及各自的质量分数。根据含量和质量分数的结果，采用数学方法计算了每个花朵部分的贡献。采用 Kruskal-Wallis H 检验和 PCA 方法进行方差分析。
计算出的花萼、花瓣、雄蕊和雌蕊、花梗和子房对木樨草苷和荭草苷产量的平均贡献率分别为 76.99%和 71.93%、9.60%和 8.33%、9.21%和 8.10%、2.17%和 6.62%以及 2.03%和 5.02%。
花朵各部分对木樨草苷和荭草苷的产量贡献不均等，其中花萼的贡献最高，与其他任何部分相比差异均有统计学意义。
