a School of Pharmaceutical Sciences, Guangzhou Higher Education Mega Center, Sun Yat-Sen University , 132 Waihuan Rd. East, 510006 Guangzhou , P.R. China.
Nat Prod Res. 2014;28(9):674-6. doi: 10.1080/14786419.2014.891111. Epub 2014 Mar 21.
Orientin and vitexin are the two main bioactive compounds in Trollius chinensis Bunge. In this study, a rapid method was established for the isolation and purification of orientin and vitexin from T. chinensis Bunge using high-speed counter-current chromatography in one step, with a solvent system of ethyl acetate-ethanol-water (4:1:5, v/v/v). A total of 9.8 mg orientin and 2.1 mg vitexin were obtained from 100 mg of the ethyl acetate extract, with purities of 99.2% and 96.0%, respectively. Their structures were identified by UV, MS and NMR. The method was efficient and convenient, which could be used for the preparative separation of orientin and vitexin from T. chinensis Bunge.
梓醇和芦丁是毛茛科植物土木香中的两种主要生物活性化合物。本研究采用高速逆流色谱一步法，以乙酸乙酯-乙醇-水（4:1:5，v/v/v）为溶剂系统，从土木香提取物中快速分离和纯化梓醇和芦丁。从 100mg 乙酸乙酯提取物中得到 9.8mg 梓醇和 2.1mg 芦丁，纯度分别为 99.2%和 96.0%。通过紫外、质谱和核磁共振对其结构进行了鉴定。该方法高效、简便，可用于土木香中梓醇和芦丁的制备分离。
