Liu Jiang-Yun, Li Sheng-Yin, Feng Jian-Yong, Sun Yan, Cai Jin-Na, Sun Xiao-Fei, Yang Shi-Lin
College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China.
J Asian Nat Prod Res. 2013;15(4):325-31. doi: 10.1080/10286020.2012.760545. Epub 2013 Apr 22.
Phytochemical investigation of ethanol extract from the flowers of Trollius chinensis Bunge resulted in the isolation of two new flavone C-glycosides (1-2), along with 10 known compounds (3-12). The structures of the new compounds were established as 6‴-(3-hydroxy-3-methylglutaroyl)-2″-O-β-d-galactopyranosyl orientin (1) and 6‴-(3-hydroxy-3-methylglutaroyl)-2″-O-β-d-galactopyranosyl vitexin (2) on the basis of various spectroscopic analysis (including different 1D and 2D NMR spectroscopies, high-resolution electrospray ionization mass spectrometry) and chemical evidences. Bioassay showed that eight flavonoids inhibited complement activation on the classic pathway in vitro, with their IC50 values ranging from 0.88 to 4.02 mM, which may contribute to the applications of the herb in treatment of acute respiratory distress syndrome, etc.
对金莲花花朵乙醇提取物进行植物化学研究，从中分离出两种新的黄酮碳苷（1 - 2），以及10种已知化合物（3 - 12）。通过各种光谱分析（包括不同的一维和二维核磁共振光谱、高分辨率电喷雾电离质谱）和化学证据，确定新化合物的结构分别为6‴-(3-羟基-3-甲基戊二酰基)-2″-O-β-D-吡喃半乳糖基荭草素（1）和6‴-(3-羟基-3-甲基戊二酰基)-2″-O-β-D-吡喃半乳糖基牡荆素（2）。生物活性测定表明，8种黄酮类化合物在体外对经典途径的补体激活具有抑制作用，其IC50值在0.88至4.02 mM之间，这可能有助于该草药在治疗急性呼吸窘迫综合征等方面的应用。
