Budkowska A, Dubreuil P, Riottot M M, Briantais M J, Pillot J
J Immunol Methods. 1987 Feb 26;97(1):77-85. doi: 10.1016/0022-1759(87)90108-6.
Pre-S2-coded sequences of the hepatitis B virus (HBV) represent important serological markers of HBV infection and elicit the antibodies essential for recovery from type B hepatitis. Monoclonal antibodies (McAbs) directed against two non-overlapping epitopes (pre-S2a and pre-S2b) of the pre-S2 protein of HBV were used to develop an enzyme immunosorbent assay (EIA). The assay was based on the solid-phase sandwich principle in which two different epitope-specific antibodies were used as immunadsorbents and as enzyme-labelled probes. The assay sensitivity was in the pg range and permitted precise quantitation of the pre-S2 sequences in sera. Using the 'site-specific' monoclonal assay we demonstrated that pre-S2a and pre-S2b epitopes are expressed on HBsAg particles of both ay and ad subtypes. The assay is the most sensitive currently available method for the detection of pre-S2 epitopes and may be used for routine immunodiagnosis of hepatitis B.
乙型肝炎病毒（HBV）的前S2编码序列是HBV感染的重要血清学标志物，可引发从乙型肝炎康复所必需的抗体。针对HBV前S2蛋白的两个不重叠表位（前S2a和前S2b）的单克隆抗体（McAbs）被用于开发一种酶免疫吸附测定（EIA）。该测定基于固相夹心原理，其中两种不同的表位特异性抗体用作免疫吸附剂和酶标记探针。测定灵敏度在皮克范围内，可对血清中的前S2序列进行精确定量。使用“位点特异性”单克隆测定，我们证明前S2a和前S2b表位在ay和ad亚型的HBsAg颗粒上均有表达。该测定是目前检测前S2表位最灵敏的方法，可用于乙型肝炎的常规免疫诊断。
